| Chapter title |
Detection of Transcriptionally Active Mycotoxin Gene Clusters: DNA Microarray
|
|---|---|
| Chapter number | 23 |
| Book title |
Mycotoxigenic Fungi
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6707-0_23 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6705-6, 978-1-4939-6707-0
|
| Authors |
Tamás Emri, Anna Zalka, István Pócsi, Emri, Tamás, Zalka, Anna, Pócsi, István |
| Abstract |
Various bioanalytical tools including DNA microarrays are frequently used to map global transcriptional changes in mycotoxin producer filamentous fungi. This effective hybridization-based transcriptomics technology helps researchers to identify genes of secondary metabolite gene clusters and record concomitant gene expression changes in these clusters initiated by versatile environmental conditions and/or gene deletions. Such transcriptional data are of great value when future mycotoxin control technologies are considered and elaborated. Giving the readers insights into RNA extraction and DNA microarray hybridization steps routinely used in our laboratories and also into the normalization and evaluation of primary gene expression data, we would like to contribute to the interlaboratory standardization of DNA microarray based transcriptomics studies being carried out in many laboratories worldwide in this important field of fungal biology. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 22% |
| Researcher | 2 | 22% |
| Student > Bachelor | 1 | 11% |
| Lecturer > Senior Lecturer | 1 | 11% |
| Student > Ph. D. Student | 1 | 11% |
| Other | 1 | 11% |
| Unknown | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 56% |
| Biochemistry, Genetics and Molecular Biology | 2 | 22% |
| Medicine and Dentistry | 1 | 11% |
| Unknown | 1 | 11% |