Chapter title |
High-Throughput Microfluidic Platform for 3D Cultures of Mesenchymal Stem Cells
|
---|---|
Chapter number | 23 |
Book title |
3D Cell Culture
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7021-6_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7019-3, 978-1-4939-7021-6
|
Authors |
Paola Occhetta, Roberta Visone, Marco Rasponi |
Editors |
Zuzana Koledova |
Abstract |
The design of innovative tools for generating physiologically relevant three-dimensional (3D) in vitro models has been recently recognized as a fundamental step to study cell responses and long-term tissue functionalities thanks to its ability to recapitulate the complexity and the dimensional scale of the cellular microenvironment, while directly integrating high-throughput and automatic screening capabilities.This chapter addresses the development of a poly(dimethylsiloxane)-based microfluidic platform to (1) generate and culture 3D cellular microaggregates under continuous flow perfusion while (2) conditioning them with different combinations/concentrations of soluble factors (i.e., growth factors, morphogens or drug molecules), in a high-throughput fashion. The proposed microfluidic system thus represents a promising tool for establishing innovative high-throughput models for drug screening, investigation of tissues morphogenesis, and optimization of tissue engineering protocols. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 37 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 12 | 32% |
Student > Bachelor | 5 | 14% |
Researcher | 5 | 14% |
Student > Doctoral Student | 4 | 11% |
Student > Master | 4 | 11% |
Other | 2 | 5% |
Unknown | 5 | 14% |
Readers by discipline | Count | As % |
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Engineering | 12 | 32% |
Biochemistry, Genetics and Molecular Biology | 6 | 16% |
Agricultural and Biological Sciences | 6 | 16% |
Philosophy | 1 | 3% |
Medicine and Dentistry | 1 | 3% |
Other | 3 | 8% |
Unknown | 8 | 22% |