| Chapter title |
Bioprinting Cartilage Tissue from Mesenchymal Stem Cells and PEG Hydrogel
|
|---|---|
| Chapter number | 28 |
| Book title |
3D Cell Culture
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-7021-6_28 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7019-3, 978-1-4939-7021-6
|
| Authors |
Gao, Guifang, Hubbell, Karen, Schilling, Arndt F., Dai, Guohao, Cui, Xiaofeng, Guifang Gao, Karen Hubbell, Arndt F. Schilling, Guohao Dai, Xiaofeng Cui Ph.D., Xiaofeng Cui |
| Editors |
Zuzana Koledova |
| Abstract |
Bioprinting based on thermal inkjet printing is one of the most attractive enabling technologies for tissue engineering and regeneration. During the printing process, cells, scaffolds , and growth factors are rapidly deposited to the desired two-dimensional (2D) and three-dimensional (3D) locations. Ideally, the bioprinted tissues are able to mimic the native anatomic structures in order to restore the biological functions. In this study, a bioprinting platform for 3D cartilage tissue engineering was developed using a commercially available thermal inkjet printer with simultaneous photopolymerization . The engineered cartilage demonstrated native zonal organization, ideal extracellular matrix (ECM ) composition, and proper mechanical properties. Compared to the conventional tissue fabrication approach, which requires extended UV exposure, the viability of the printed cells with simultaneous photopolymerization was significantly higher. Printed neocartilage demonstrated excellent glycosaminoglycan (GAG) and collagen type II production, which was consistent with gene expression profile. Therefore, this platform is ideal for anatomic tissue engineering with accurate cell distribution and arrangement. |
X Demographics
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Demographic breakdown
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Mendeley readers
Geographical breakdown
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Demographic breakdown
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|---|---|---|
| Researcher | 12 | 16% |
| Student > Master | 10 | 13% |
| Student > Bachelor | 9 | 12% |
| Student > Ph. D. Student | 9 | 12% |
| Student > Postgraduate | 4 | 5% |
| Other | 7 | 9% |
| Unknown | 24 | 32% |
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| Medicine and Dentistry | 3 | 4% |
| Other | 7 | 9% |
| Unknown | 26 | 35% |