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Ethylene Signaling

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Cover of 'Ethylene Signaling'

Table of Contents

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    Book Overview
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    Chapter 1 Gas Chromatography-Based Ethylene Measurement of Arabidopsis Seedlings
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    Chapter 2 Plant Ethylene Detection Using Laser-Based Photo-Acoustic Spectroscopy
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    Chapter 3 Treatment of Plants with Gaseous Ethylene and Gaseous Inhibitors of Ethylene Action
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    Chapter 4 Analysis of 1-Aminocyclopropane-1-Carboxylic Acid Uptake Using a Protoplast System
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    Chapter 5 Escherichia coli-Based Expression and In Vitro Activity Assay of 1-Aminocyclopropane-1-Carboxylate (ACC) Synthase and ACC Oxidase
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    Chapter 6 Assay Methods for ACS Activity and ACS Phosphorylation by MAP Kinases In Vitro and In Vivo
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    Chapter 7 Analysis of Ethylene Receptors: Ethylene-Binding Assays
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    Chapter 8 Analysis of Ethylene Receptors: Assay for Histidine Kinase Activity
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    Chapter 9 Analysis of Ethylene Receptor Interactions by Co-immunoprecipitation Assays
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    Chapter 10 Localization of the Ethylene-Receptor Signaling Complex to the Endoplasmic Reticulum: Analysis by Two-Phase Partitioning and Density-Gradient Centrifugation
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    Chapter 11 Kinase Assay for CONSTITUTIVE TRIPLE RESPONSE 1 (CTR1) in Arabidopsis thaliana
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    Chapter 12 Circular Dichroism and Fluorescence Spectroscopy to Study Protein Structure and Protein–Protein Interactions in Ethylene Signaling
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    Chapter 13 The Triple Response Assay and Its Use to Characterize Ethylene Mutants in Arabidopsis
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    Chapter 14 Time-Lapse Imaging to Examine the Growth Kinetics of Arabidopsis Seedlings in Response to Ethylene
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    Chapter 15 Inhibitors of Ethylene Biosynthesis and Signaling
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    Chapter 16 Analysis of Growth and Molecular Responses to Ethylene in Etiolated Rice Seedlings
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    Chapter 17 Love Me Not Meter: A Sensor Device for Detecting Petal Detachment Forces in Arabidopsis thaliana
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    Chapter 18 Effects of Ethylene on Seed Germination of Halophyte Plants Under Salt Stress
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    Chapter 19 Assessing Attraction of Nematodes to Host Roots Using Pluronic Gel Medium
Attention for Chapter 4: Analysis of 1-Aminocyclopropane-1-Carboxylic Acid Uptake Using a Protoplast System
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Chapter title
Analysis of 1-Aminocyclopropane-1-Carboxylic Acid Uptake Using a Protoplast System
Chapter number 4
Book title
Ethylene Signaling
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6854-1_4
Pubmed ID
Book ISBNs
978-1-4939-6852-7, 978-1-4939-6854-1
Authors

Won-Yong Song, Sumin Lee, Moon-Soo Soh

Editors

Brad M. Binder, G. Eric Schaller

Abstract

1-Aminocyclopropane-1-carboxylic acid (ACC) is a biosynthetic precursor of ethylene. The movement of ACC across the plasma membrane (PM) has been implicated in various physiological contexts during environmental adaptation and differentiation in higher plants. A PM-localized transporter in Arabidopsis thaliana, LYSINE HISTIDINE TRANSPORTER1 (LHT1) participates in the uptake of ACC, implicating a class of amino-acid transporters in the transport of ACC. Here, we describe the method for assaying uptake of ACC into the plant cells using an Arabidopsis mesophyll protoplast system.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 25%
Student > Ph. D. Student 1 25%
Unknown 2 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 25%
Agricultural and Biological Sciences 1 25%
Unknown 2 50%