Chapter title |
Analysis of Ethylene Receptor Interactions by Co-immunoprecipitation Assays
|
---|---|
Chapter number | 9 |
Book title |
Ethylene Signaling
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6854-1_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6852-7, 978-1-4939-6854-1
|
Authors |
Zhiyong Gao, G. Eric Schaller |
Editors |
Brad M. Binder, G. Eric Schaller |
Abstract |
Ethylene receptors are predominantly localized to the endoplasmic reticulum (ER) membrane, and coordinate ethylene signal output through protein-protein interactions with each other and additional signaling components. Here, we describe a co-immunoprecipitation (Co-IP) assay based on the use of the Tandem Affinity Purification (TAP) tag to examine the interactions of ethylene receptors in plant extracts. Human IgG-agarose beads are used to pull down TAP-tagged versions of the protein of interest from detergent extracts of Arabidopsis membranes, and the precipitate then is analyzed immunologically for co-purification of the ethylene receptors. This method has been successfully used to examine interactions of the receptors with each other as well as with the Raf-like kinase CTR1. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 20% |
Researcher | 1 | 20% |
Student > Doctoral Student | 1 | 20% |
Unknown | 2 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 40% |
Biochemistry, Genetics and Molecular Biology | 1 | 20% |
Unknown | 2 | 40% |