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Vaccines for Invasive Fungal Infections

Overview of attention for book
Cover of 'Vaccines for Invasive Fungal Infections'

Table of Contents

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    Book Overview
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    Chapter 1 Mouse Immunization with Radioattenuated Yeast Cells of Paracoccidioides brasiliensis
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    Chapter 2 Heat-Killed Yeast as a Pan-Fungal Vaccine
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    Chapter 3 Immunoinformatics as a Tool for New Antifungal Vaccines
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    Chapter 4 Rational Design of T Lymphocyte Epitope-Based Vaccines Against Coccidioides Infection
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    Chapter 5 Identification of Fungal T Cell Epitopes by Mass Spectrometry-Based Proteomics
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    Chapter 6 Intranasal Antifungal Vaccination Using DNA-Transfected Dendritic Cells
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    Chapter 7 DNAhsp65 Vaccine as Therapy against Paracoccidioidomycosis
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    Chapter 8 Idiotypic Antifungal Vaccination: Immunoprotection by Antiidiotypic Antibiotic Antibodies
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    Chapter 9 Peptide Vaccine Against Paracoccidioidomycosis
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    Chapter 10 Methodology for Anti-Cryptococcal Vaccine Development
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    Chapter 11 Beta-Glucan Particles as Vaccine Adjuvant Carriers
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    Chapter 12 Th1-Inducing Agents in Prophylaxis and Therapy for Paracoccidioidomycosis
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    Chapter 13 Nanoparticle-Based Mycosis Vaccine
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    Chapter 14 Yeast Expressing Gp43 Protein as a Vaccine Against Paracoccidioides brasiliensis Infection
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    Chapter 15 Vaccination with Phage-Displayed Antigenic Epitope
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    Chapter 16 Preparation of an Oral Vaccine by Proteome Analysis and Molecular Display Technology
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    Chapter 17 Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System
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    Chapter 18 Endpoint Assessment in Rabbit Models of Invasive Pulmonary Aspergillosis and Mucormycosis
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    Chapter 19 CD4+ T Cells Mediate Aspergillosis Vaccine Protection
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    Chapter 20 T-Cell-Mediated Cross-Protective Immunity
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    Chapter 21 Assessment of Post-Vaccination Phagocytic Activation Using Candida albicans Killing Assays
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    Chapter 22 Immunization with Antigen-Pulsed Dendritic Cells Against Highly Virulent Cryptococcus gattii Infection: Analysis of Cytokine-Producing T Cells
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    Chapter 23 Testing Antifungal Vaccines in an Animal Model of Invasive Candidiasis and in Human Mucosal Candidiasis
Attention for Chapter 17: Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System
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Chapter title
Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System
Chapter number 17
Book title
Vaccines for Invasive Fungal Infections
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7104-6_17
Pubmed ID
28584995
Book ISBNs
978-1-4939-7103-9, 978-1-4939-7104-6
Authors

Chi Zhang, Ling Lu, Zhang, Chi, Lu, Ling

Editors

Markus Kalkum, Margarita Semis

Abstract

As one of the most common airborne fungal pathogenic species, Aspergillus fumigatus infection is the increasingly fatal threat to immunocompromised patients worldwide. Setting up an efficient live-cell pathogen-labeling system will give insight into the dynamic process of the pathogen invasion in host, which offers us opportunities to explore the pathogenesis of A. fumigatus. In this chapter, we have described an efficient CRISPR-Cas9 system, which enables a precise in situ tag-insertion of an exogenous GFP tag at the predicted site with or without marker insertion in A. fumigatus. According to the detectable proportional fluorescence intensity, it is possible to in vivo track the A. fumigatus infection and to assess the fungal burden in relative organs.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 18%
Unspecified 1 9%
Other 1 9%
Professor 1 9%
Student > Bachelor 1 9%
Other 2 18%
Unknown 3 27%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 36%
Biochemistry, Genetics and Molecular Biology 3 27%
Unspecified 1 9%
Immunology and Microbiology 1 9%
Engineering 1 9%
Other 0 0%
Unknown 1 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 March 2018.
All research outputs
#18,555,330
of 22,981,247 outputs
Outputs from Methods in molecular biology
#7,946
of 13,148 outputs
Outputs of similar age
#311,308
of 421,126 outputs
Outputs of similar age from Methods in molecular biology
#693
of 1,074 outputs
Altmetric has tracked 22,981,247 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,148 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,126 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.

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