Chapter title |
Assaying Radiosensitivity of Ataxia-Telangiectasia
|
---|---|
Chapter number | 1 |
Book title |
ATM Kinase
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6955-5_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6953-1, 978-1-4939-6955-5
|
Authors |
Hailiang Hu Ph.D., Shareef Nahas, Richard A. Gatti M.D., Hailiang Hu, Richard A. Gatti, Hu, Hailiang, Nahas, Shareef, Gatti, Richard A. |
Editors |
Sergei V. Kozlov |
Abstract |
Ataxia-Telangiectasia (A-T) is a prototypical genomic instability disorder with multi-organ deficiency and it is caused by the defective function of a single gene, ATM (Ataxia-Telangiectasia Mutated). Radiosensitivity, among the pleiotropic symptoms of A-T, reflects the basic physiological functions of ATM protein in the double strand break (DSB)-induced DNA damage response (DDR) and also restrains A-T patients from the conventional radiation therapy for their lymphoid malignancy. In this chapter, we describe two methods that have been developed in our lab to assess the radiosensitivity of A-T patients: (1) Colony Survival Assay (CSA) and (2) Flow Cytometry of phospho-SMC1 (FC-pSMC1). The establishment of these more rapid and reliable functional assays to measure the radiosensitivity, exemplified by A-T, would facilitate the diagnosis of other genomic instability genetic disorders as well as help the treatment options for most radiosensitive patients. |
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Mendeley readers
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Student > Bachelor | 1 | 14% |
Researcher | 1 | 14% |
Student > Doctoral Student | 1 | 14% |
Unknown | 2 | 29% |
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Unknown | 1 | 14% |