Chapter title |
Monitoring DNA Repair Consequences of ATM Signaling Using Simultaneous Fluorescent Readouts
|
---|---|
Chapter number | 24 |
Book title |
ATM Kinase
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6955-5_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6953-1, 978-1-4939-6955-5
|
Authors |
Adrian Wiegmans |
Editors |
Sergei V. Kozlov |
Abstract |
The repair of deleterious DNA double strand breaks is required to maintain genome integrity. The efficacy in which this occurs relies upon the available machinery and is guided by factors that include cell cycle status, availability of donor template, and the local chromosome structure. Therefore at a single DNA breakpoint there are different outcomes that can occur. The Traffic light reporter (TLR) assay protocol is a dual fluorescent readout that has the ability to monitor simultaneous homologous recombination and non-homologous end joining activity in response to DNA damage. This provides insight to determine the upstream functionality of either pathway mediated through ATM. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 40% |
Professor > Associate Professor | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 60% |
Chemistry | 1 | 20% |
Unknown | 1 | 20% |