Chapter title |
Repurposing CRISPR System for Transcriptional Activation
|
---|---|
Chapter number | 10 |
Book title |
RNA Activation
|
Published in |
Advances in experimental medicine and biology, June 2017
|
DOI | 10.1007/978-981-10-4310-9_10 |
Pubmed ID | |
Book ISBNs |
978-9-81-104309-3, 978-9-81-104310-9
|
Authors |
Meng Chen, Lei Stanley Qi |
Editors |
Long-Cheng Li |
Abstract |
In recent years, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has become the most popular one for genome editing. When the nuclease domains of Cas9 protein are mutated into deactivated form (dCas9), CRISPR/dCas9 still retains the ability to bind the targeted DNA sequence, but loses the endonuclease cleavage activity. Taking advantage of the characteristics of this engineered nuclease inactive Cas9, the CRISPR/dCas system has been repurposed into versatile RNA-guided, DNA-targeting platforms, such as genome imaging, gene regulation, and epigenetic modification. Specifically, fusion of dCas9 with activation domains allows specific and efficient transcriptional activation on a genome-wide scale among diverse organisms. The purpose of this chapter is to review most important the recently published literature on CRISPR/dCas9-based transcriptional activation systems. Compared with the conventional approaches for enhancement of the expression of specific genes of interest, CRISPR/Cas9-based system has emerged as a promising technology for genome regulation, allowing specificity, convenience, robustness, and scalability for endogenous gene activation. |
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