Chapter title |
Delivery of Cargo to Lysosomes Using GNeosomes
|
---|---|
Chapter number | 9 |
Book title |
Lysosomes
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6934-0_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6932-6, 978-1-4939-6934-0
|
Authors |
Kristina M. Hamill, Ezequiel Wexselblatt, Wenyong Tong, Jeffrey D. Esko, Yitzhak Tor |
Editors |
Karin Öllinger, Hanna Appelqvist |
Abstract |
Liposomes have been used to improve the intracellular delivery of a variety of cargos. Encapsulation of cargos in liposomes leads to improved plasma half-lives and minimized degradation. Here, we present a method for improving the selective delivery of liposomes to the lysosomes using a guanidinylated neomycin (GNeo) transporter. The method for synthesizing GNeo-lipids, incorporating them into liposomes, and the enhanced lysosomal delivery of encapsulated cargo are presented. GNeo-liposomes, termed GNeosomes, are capable of delivering a fluorescent dye to the lysosomes of Chinese hamster ovary cells as shown using confocal microscopy. GNeosomes can also be used to deliver therapeutic quantities of lysosomal enzymes to fibroblasts isolated from patients with a lysosomal storage disorder. |
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