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Lysosomes

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Cover of 'Lysosomes'

Table of Contents

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    Book Overview
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    Chapter 1 SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS. - PubMed - NCBI
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    Chapter 2 Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics
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    Chapter 3 Analysis of N- and O-Glycosylation of Lysosomal Glycoproteins
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    Chapter 4 Analyzing Lysosome-Related Organelles by Electron Microscopy
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    Chapter 5 Microscopic Analysis of Lysosomal Membrane Permeabilization
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    Chapter 6 Quantitative Co-Localization and Pattern Analysis of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets
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    Chapter 7 Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues
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    Chapter 8 Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy-Dependent Recovery
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    Chapter 9 Delivery of Cargo to Lysosomes Using GNeosomes
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    Chapter 10 Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles
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    Chapter 11 Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells
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    Chapter 12 Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry
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    Chapter 13 Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes
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    Chapter 14 Using the MEROPS Database for Investigation of Lysosomal Peptidases, Their Inhibitors, and Substrates
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    Chapter 15 Next-Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of LysoPlex
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    Chapter 16 Gelatin Zymography Using Leupeptin for the Detection of Various Cathepsin L Forms
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    Chapter 17 Methods for Determination of α-Glycosidase, β-Glycosidase, and α-Galactosidase Activities in Dried Blood Spot Samples
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    Chapter 18 Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi
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    Chapter 19 Lysosomal Biology in Cancer
Attention for Chapter 12: Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry
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Chapter title
Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry
Chapter number 12
Book title
Lysosomes
Published in
Methods in molecular biology, April 2017
DOI 10.1007/978-1-4939-6934-0_12
Pubmed ID
28456984
Book ISBNs
978-1-4939-6932-6, 978-1-4939-6934-0
Authors

Anna L. Södergren, Sofia Ramström

Editors

Karin Öllinger, Hanna Appelqvist

Abstract

Flow cytometry is a method that allows high throughput analysis of individual cells in suspension. By inclusion of fluorescently labelled antibodies, it is possible to analyze the abundance of one or more surface antigens, such as LAMP-1, without prior lysis of cells. Here we describe the special considerations required for the investigation of lysosomal exocytosis from platelets analyzed with flow cytometry.

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Mendeley readers

The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 1 100%
Readers by discipline Count As %
Medicine and Dentistry 1 100%

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