| Chapter title |
Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics
|
|---|---|
| Chapter number | 2 |
| Book title |
Lysosomes
|
| Published in |
Methods in molecular biology, April 2017
|
| DOI | 10.1007/978-1-4939-6934-0_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6932-6, 978-1-4939-6934-0
|
| Authors |
Bilgin, Mesut, Nylandsted, Jesper, Jäättelä, Marja, Maeda, Kenji, Mesut Bilgin, Jesper Nylandsted, Marja Jäättelä, Kenji Maeda |
| Editors |
Karin Öllinger, Hanna Appelqvist |
| Abstract |
Recent studies have illuminated novel roles of lysosomes that go far beyond simple catabolism and function in the coordination of cellular metabolism and signaling. Promising therapeutic strategies emerge from knowledge in the molecular mechanisms and physiological roles of lipid metabolism in lysosomes. Global monitoring of the function and dysregulation of lysosomal lipid metabolism requires a methodology that resolves the complexity of lysosomal lipidome by quantitatively detecting hundreds of lipid species of diverse physicochemical properties. We describe here a detailed protocol that couples isolation of superparamagnetic iron dextran-loaded lysosomes from cultured mammalian cell lines with quantitative mass spectrometry-based shotgun lipidomics. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 26% |
| Student > Ph. D. Student | 3 | 16% |
| Student > Postgraduate | 2 | 11% |
| Professor > Associate Professor | 2 | 11% |
| Student > Master | 1 | 5% |
| Other | 3 | 16% |
| Unknown | 3 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 26% |
| Agricultural and Biological Sciences | 4 | 21% |
| Nursing and Health Professions | 1 | 5% |
| Psychology | 1 | 5% |
| Medicine and Dentistry | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 6 | 32% |