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Mendeley readers
| Chapter title |
User Guide for the LORE1 Insertion Mutant Resource
|
|---|---|
| Chapter number | 2 |
| Book title |
Plant Genomics
|
| Published in |
Methods in molecular biology, April 2017
|
| DOI | 10.1007/978-1-4939-7003-2_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7001-8, 978-1-4939-7003-2
|
| Authors |
Terry Mun, Anna Małolepszy, Niels Sandal, Jens Stougaard, Stig U. Andersen |
| Editors |
Wolfgang Busch |
| Abstract |
Lotus japonicus is a model legume used in the study of plant-microbe interactions, especially in the field of biological nitrogen fixation due to its ability to enter into a symbiotic relationship with a soil bacterium, Mesorhizobium loti. The LORE1 mutant population is a valuable resource for reverse genetics in L. japonicus due to its non-transgenic nature, high tagging efficiency, and low copy count. Here, we outline a workflow for identifying, ordering, and establishing homozygous LORE1 mutant lines for a gene of interest, LjFls2, including protocols for growth and genotyping of a segregating LORE1 population. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 30% |
| Researcher | 3 | 30% |
| Professor | 2 | 20% |
| Student > Master | 1 | 10% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 40% |
| Agricultural and Biological Sciences | 4 | 40% |
| Unknown | 2 | 20% |