Chapter title |
Genome-Wide Profiling of Histone Modifications and Histone Variants in Arabidopsis thaliana and Marchantia polymorpha
|
---|---|
Chapter number | 7 |
Book title |
Plant Genomics
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-7003-2_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7001-8, 978-1-4939-7003-2
|
Authors |
Yelagandula, Ramesh, Osakabe, Akihisa, Axelsson, Elin, Berger, Frederic, Kawashima, Tomokazu, Ramesh Yelagandula, Akihisa Osakabe, Elin Axelsson, Frederic Berger, Tomokazu Kawashima |
Editors |
Wolfgang Busch |
Abstract |
Histone modifications and histone variants barcode the genome and play major roles in epigenetic regulations. Chromatin immunoprecipitation (ChIP) coupled with next-generation sequencing (NGS) is a well-established method to investigate the landscape of epigenetic marks at a genomic level. Here, we describe procedures for conducting ChIP, subsequent NGS library construction, and data analysis on histone modifications and histone variants in Arabidopsis thaliana. We also describe an optimized nuclear isolation procedure to prepare chromatin for ChIP in the liverwort, Marchantia polymorpha, which is the emerging model plant ideal for evolutionary studies. |
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