Chapter title |
Circular Chromosome Conformation Capture in Plants
|
---|---|
Chapter number | 6 |
Book title |
Plant Genomics
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-7003-2_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7001-8, 978-1-4939-7003-2
|
Authors |
Grob, Stefan, Stefan Grob |
Editors |
Wolfgang Busch |
Abstract |
The study of nuclear architecture promises novel insights into genome function and regulation. Hereby, quantitative methods based on chromosome conformation capture (3C) revolutionized the field, as they allow accurate and unbiased characterization of 3D genome organization of genomic regions of interest. The choice of the appropriate 3C derivate is crucial to acquire results suited for a specific research question. Circular 3C (4C) is the method of choice to study the genome-wide 3D architecture of a specific genomic region of interest. Here, I present a robust 4C protocol, established in Arabidopsis thaliana, which can be employed by any experienced molecular biologist and is applicable in various other plant species. |
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