Chapter title |
Colorimetric Detection of Acetyl Xylan Esterase Activities
|
---|---|
Chapter number | 5 |
Book title |
Protein-Carbohydrate Interactions
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6899-2_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6898-5, 978-1-4939-6899-2
|
Authors |
Galina Mai-Gisondi, Emma R. Master |
Editors |
D. Wade Abbott, Alicia Lammerts van Bueren |
Abstract |
Colorimetric detection of reaction products is typically preferred for initial surveys of acetyl xylan esterase (AcXE) activity. This chapter will describe common colorimetric methods, and variations thereof, for measuring AcXE activities on commercial, synthesized, and natural substrates. Whereas assays using pNP-acetate, α-naphthyl acetate, and 4-methylumbelliferyl acetate (4MUA) are emphasized, common methods used to measure AcXE activity towards carbohydrate analogs (e.g., acetylated p-nitrophenyl β-D-xylopyranosides) and various acetylated xylans are also described. Strengths and limitations of the colorimetric assays are highlighted. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 25% |
Researcher | 2 | 17% |
Student > Ph. D. Student | 2 | 17% |
Professor | 1 | 8% |
Student > Doctoral Student | 1 | 8% |
Other | 1 | 8% |
Unknown | 2 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 50% |
Agricultural and Biological Sciences | 2 | 17% |
Chemistry | 1 | 8% |
Unknown | 3 | 25% |