Chapter title |
System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates
|
---|---|
Chapter number | 8 |
Book title |
Protein Terminal Profiling
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6850-3_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6849-7, 978-1-4939-6850-3
|
Authors |
Zon W. Lai, Oliver Schilling |
Editors |
Oliver Schilling |
Abstract |
Clinical tissues are used for histopathological diagnosis of many diseases including immunostaining and morphology subtyping as well as in molecular research such as for the analyses of DNA, RNA, and proteins. Formalin fixation and paraffin embedment (FFPE) of tissue specimens is routinely used for preserving clinical tissues for long-term storage, allowing histopathological diagnosis of many diseases. As such, FFPE tissues currently represent the most comprehensive collection of all clinical specimens, allowing great source of material for research opportunity, possibly due to the concern of protein integrity from antigen retrieval from fixation process. Hence, to date, very few studies have used FFPE specimens to look at the profiling of protein termini. Nevertheless in the field of protease research, the protein amino termini are particularly useful for the system-wide identification of substrates and for the characterization of protease-mediated cleavage sites . In this chapter, we outline a robust methodology for the extraction of proteins from FFPE specimens for the enrichment of protein amino termini. This approach enriches endogenous protein N-termini by removal of internal peptides using synthetic polymers of hyperbranched polyglycerol aldehyde. As a result, protein amino termini are analyzed using mass spectrometers to elucidate the biological regulation of protease-substrate interactions in healthy and diseased tissues. |
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