| Chapter title |
Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification
|
|---|---|
| Chapter number | 6 |
| Book title |
Protein Terminal Profiling
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6850-3_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6849-7, 978-1-4939-6850-3
|
| Authors |
Benoit Westermann, Alvaro Sebastian Vaca Jacome, Magali Rompais, Christine Carapito, Christine Schaeffer-Reiss |
| Editors |
Oliver Schilling |
| Abstract |
The study of the N-terminome and the precise identification of proteolytic processing events are key in biology. Dedicated methodologies have been developed as the comprehensive characterization of the N-terminome can hardly be achieved by standard proteomics methods. In this context, we have set up a trimethoxyphenyl phosphonium (TMPP) labeling approach that allows the characterization of both N-terminal and internal digestion peptides in a single experiment. This latter point is a major advantage of our strategy as most N-terminomics methods rely on the enrichment of N-terminal peptides and thus exclude internal peptides.We have implemented a double heavy/light TMPP labeling and an automated data validation workflow that make our doublet N-terminal oriented proteomics (dN-TOP) strategy efficient for high-throughput N-terminome analysis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 23 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 6 | 26% |
| Professor > Associate Professor | 2 | 9% |
| Student > Master | 2 | 9% |
| Unspecified | 1 | 4% |
| Professor | 1 | 4% |
| Other | 2 | 9% |
| Unknown | 9 | 39% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 22% |
| Mathematics | 2 | 9% |
| Agricultural and Biological Sciences | 2 | 9% |
| Unspecified | 1 | 4% |
| Computer Science | 1 | 4% |
| Other | 2 | 9% |
| Unknown | 10 | 43% |