Chapter title |
Quantitative Real-Time PCR: Recent Advances
|
---|---|
Chapter number | 15 |
Book title |
Clinical Applications of PCR
|
Published in |
Methods in molecular biology, February 2016
|
DOI | 10.1007/978-1-4939-3360-0_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3358-7, 978-1-4939-3360-0
|
Authors |
Singh, Charanjeet, Roy-Chowdhuri, Sinchita, Charanjeet Singh, Sinchita Roy-Chowdhuri |
Editors |
Rajyalakshmi Luthra, Rajesh R. Singh, Keyur P. Patel |
Abstract |
Quantitative real-time polymerase chain reaction is a technique for simultaneous amplification and product quantification of a target DNA as the process takes place in real time in a "closed-tube" system. Although this technique can provide an absolute quantification of the initial template copy number, quantification relative to a control sample or second sequence is typically adequate. The quantification process employs melting curve analysis and/or fluorescent detection systems and can provide amplification and genotyping in a relatively short time. Here we describe the properties and uses of various fluorescent detection systems used for quantification. |
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