Chapter title |
Polymerase Chain Reaction Diagnosis of Leishmaniasis: A Species-Specific Approach
|
---|---|
Chapter number | 11 |
Book title |
Clinical Applications of PCR
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3360-0_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3358-7, 978-1-4939-3360-0
|
Authors |
González-Marcano, Eglys, Kato, Hirotomo, Concepción, Juan Luis, Márquez, María Elizabeth, Mondolfi, Alberto Paniz, Eglys González-Marcano, Hirotomo Kato, Juan Luis Concepción, María Elizabeth Márquez, Alberto Paniz Mondolfi |
Editors |
Rajyalakshmi Luthra, Rajesh R. Singh, Keyur P. Patel |
Abstract |
Leishmaniasis is an infectious disease caused by protozoan parasites of the genus Leishmania which are transmitted to humans through bites of infected sand flies. The variable clinical manifestations and the evolution of the disease are determined by the infecting species. Recognition at a species level is of utmost importance since this greatly impacts therapy decision making as well as predicts outcome for the disease. This chapter describes the application of polymerase chain reaction (PCR) in the detection of Leishmania parasites across the disease spectrum, including protocols for sample collection and transportation, genomic material extraction, and target amplification methods with special emphasis on PCR amplification of the cytochrome b gene for Leishmania spp. species identification. |
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Mendeley readers
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Student > Bachelor | 2 | 20% |
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Student > Master | 1 | 10% |
Researcher | 1 | 10% |
Other | 0 | 0% |
Unknown | 1 | 10% |
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Immunology and Microbiology | 1 | 10% |
Other | 1 | 10% |
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