| Chapter title |
Rapid Selection of RNA Aptamers that Activate Fluorescence of Small Molecules
|
|---|---|
| Chapter number | 17 |
| Book title |
Synthetic Antibodies
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6857-2_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6855-8, 978-1-4939-6857-2
|
| Authors |
Grigory S. Filonov |
| Editors |
Thomas Tiller |
| Abstract |
RNA aptamers can serve as valuable tools for studying and manipulating live cells. Fluorescent aptamers are the ones that bind to and turn on fluorescence of small-molecule dyes (fluorogens). Similarly to fluorescent proteins, fluorescent RNA aptamers can be used to image spatial and temporal RNA dynamics in live cells. Additionally, these aptamers can serve as a basis for engineering genetically encoded fluorescent biosensors. This chapter presents a protocol for rapid and efficient screening of RNA aptamer libraries to isolate fluorescent aptamers. The protocol describes how to design, clone, and express RNA aptamer library in bacterial cells and how to screen the bacteria to find aptamers with the desired fluorescent properties. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 50% |
| Student > Postgraduate | 2 | 20% |
| Student > Ph. D. Student | 1 | 10% |
| Unknown | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 40% |
| Chemistry | 2 | 20% |
| Immunology and Microbiology | 1 | 10% |
| Agricultural and Biological Sciences | 1 | 10% |
| Unknown | 2 | 20% |