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Mendeley readers
| Chapter title |
Replacement of Antibodies in Pseudo-ELISAs: Molecularly Imprinted Nanoparticles for Vancomycin Detection
|
|---|---|
| Chapter number | 25 |
| Book title |
Synthetic Antibodies
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6857-2_25 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6855-8, 978-1-4939-6857-2
|
| Authors |
Francesco Canfarotta, Katarzyna Smolinska-Kempisty, Sergey Piletsky |
| Editors |
Thomas Tiller |
| Abstract |
The enzyme-linked immunosorbent assay (ELISA) is a widely employed analytical test used to quantify a given molecule. It relies on the use of specific antibodies, linked to an enzyme, to target the desired molecule. The reaction between the enzyme and its substrate gives rise to the analytical signal that can be quantified. Thanks to their robustness and low cost, molecularly imprinted polymer nanoparticles (nanoMIPs) are a viable alternative to antibodies. Herein, we describe the synthesis of nanoMIPs imprinted for vancomycin and their subsequent application in an ELISA-like format for direct replacement of antibodies. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 22% |
| Student > Ph. D. Student | 3 | 17% |
| Student > Doctoral Student | 2 | 11% |
| Researcher | 2 | 11% |
| Lecturer | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 5 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 6 | 33% |
| Biochemistry, Genetics and Molecular Biology | 2 | 11% |
| Chemical Engineering | 1 | 6% |
| Agricultural and Biological Sciences | 1 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Other | 2 | 11% |
| Unknown | 5 | 28% |