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Mendeley readers
Chapter title |
A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteins
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---|---|
Chapter number | 14 |
Book title |
Synthetic Antibodies
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Published in |
Methods in molecular biology, March 2017
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DOI | 10.1007/978-1-4939-6857-2_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6855-8, 978-1-4939-6857-2
|
Authors |
Wouter P. R. Verdurmen, Marigona Mazlami, Andreas Plückthun |
Editors |
Thomas Tiller |
Abstract |
The efficient delivery of external proteins from the external milieu to the cytosol of mammalian cells has great potential for both scientific investigations and future therapies. However, when assessing the cellular uptake of proteins, it is often difficult to distinguish between proteins that are stuck in the endosomes and those that have escaped into the cytosol. Here, we describe a method employing the prokaryotic enzyme biotin ligase that overcomes this problem and which can be employed for a highly sensitive quantification of cytosolic protein delivery. |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 20% |
Other | 2 | 13% |
Student > Bachelor | 2 | 13% |
Student > Doctoral Student | 1 | 7% |
Unspecified | 1 | 7% |
Other | 4 | 27% |
Unknown | 2 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 33% |
Chemistry | 4 | 27% |
Biochemistry, Genetics and Molecular Biology | 2 | 13% |
Business, Management and Accounting | 1 | 7% |
Unspecified | 1 | 7% |
Other | 0 | 0% |
Unknown | 2 | 13% |