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Oat

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Cover of 'Oat'

Table of Contents

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    Book Overview
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    Chapter 1 Fluorescence In Situ Hybridization in Oat
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    Chapter 2 Oat Doubled Haploids Following Maize Pollination
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    Chapter 3 Wide Hybridization Between Oat and Pearl Millet
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    Chapter 4 Protocol for Producing Synthetic Polyploid Oats
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    Chapter 5 Manipulation of Oat Protoplasts for Transient Expression Assays
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    Chapter 6 Oat Anther Culture and Use of DH-Lines for Genetic Mapping
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    Chapter 7 Agrobacterium-Mediated Transformation of Leaf Base Segments
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    Chapter 8 Chromatographic Methods to Evaluate Nutritional Quality in Oat
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    Chapter 9 Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry
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    Chapter 10 Multiplex Dipstick Immunoassay for Semiquantitative Determination of Fusarium Mycotoxins in Oat
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    Chapter 11 Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat
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    Chapter 12 M13-Tailed Simple Sequence Repeat (SSR) Markers in Studies of Genetic Diversity and Population Structure of Common Oat Germplasm
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    Chapter 13 Genotyping-by-Sequencing and Its Application to Oat Genomic Research
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    Chapter 14 Genome-Wide Association Analysis Using R
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    Chapter 15 De Novo Transcriptome Assembly in Polyploid Species
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    Chapter 16 Isolation of Oat (Avena sativa L.) Total Proteins and Their Prolamin Fractions for 2D Electrophoresis
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    Chapter 17 2-DE Separation and Identification of Oat (Avena sativa L.) Proteins and Their Prolamin Fractions
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    Chapter 18 Selected Bioinformatic Tools and MS (MALDI-TOF, PMF) Techniques Used in the Strategy for the Identification of Oat Proteins After 2-DE
Attention for Chapter 17: 2-DE Separation and Identification of Oat (Avena sativa L.) Proteins and Their Prolamin Fractions
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Chapter title
2-DE Separation and Identification of Oat (Avena sativa L.) Proteins and Their Prolamin Fractions
Chapter number 17
Book title
Oat
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6682-0_17
Pubmed ID
28132155
Book ISBNs
978-1-4939-6680-6, 978-1-4939-6682-0
Authors

Dorota Nałęcz, Iwona Szerszunowicz, Marta Dziuba, Piotr Minkiewicz

Editors

Sebastian Gasparis

Abstract

At present two-dimensional polyacrylamide gel electrophoresis (2-DE) is the most widely used proteomic tool, which enables simultaneous separation of even thousands of proteins with a high degree of resolution. The quality of 2-DE separation depends on the type of biological material used as a protein source. The presence of interfering compounds (e.g., phenols, as it is the fact in plant material including oat seeds) impedes 2-DE run. With the use of this technique it is possible to analyze the complex protein mixtures, characteristic protein fractions, as well as individual proteins.The purpose of this chapter is to describe the 2-DE technique (the separate stages of the first and the second dimension) for determining the oat protein composition (oat seed proteome), separation and preliminary identification of oat prolamin fractions. Electrophoretically separated proteins are identified on the basis of pI markers (identifying the location of both ends of an IPG strip) and on 2D SDS-PAGE standards. The gel images of oat proteins are analyzed with the help of ImageMaster 2D Platinum 6.0 program (Amersham Bioscience, part of GE Healthcare, Uppsala, Sweden). It allows finding unique spot identifiers for the occurrence of oat prolamin fractions in oat total proteins. The characteristic spots of similar shape and intensity (anchoring spots) and characteristic groups of spots can be searched for the purpose of identification.

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Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 67%
Other 1 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 33%
Unknown 2 67%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 February 2018.
All research outputs
#18,530,362
of 22,952,268 outputs
Outputs from Methods in molecular biology
#7,935
of 13,137 outputs
Outputs of similar age
#311,015
of 420,594 outputs
Outputs of similar age from Methods in molecular biology
#733
of 1,155 outputs
Altmetric has tracked 22,952,268 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,137 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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