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Oat

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Cover of 'Oat'

Table of Contents

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    Book Overview
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    Chapter 1 Fluorescence In Situ Hybridization in Oat
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    Chapter 2 Oat Doubled Haploids Following Maize Pollination
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    Chapter 3 Wide Hybridization Between Oat and Pearl Millet
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    Chapter 4 Protocol for Producing Synthetic Polyploid Oats
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    Chapter 5 Manipulation of Oat Protoplasts for Transient Expression Assays
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    Chapter 6 Oat Anther Culture and Use of DH-Lines for Genetic Mapping
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    Chapter 7 Agrobacterium-Mediated Transformation of Leaf Base Segments
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    Chapter 8 Chromatographic Methods to Evaluate Nutritional Quality in Oat
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    Chapter 9 Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry
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    Chapter 10 Multiplex Dipstick Immunoassay for Semiquantitative Determination of Fusarium Mycotoxins in Oat
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    Chapter 11 Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat
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    Chapter 12 M13-Tailed Simple Sequence Repeat (SSR) Markers in Studies of Genetic Diversity and Population Structure of Common Oat Germplasm
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    Chapter 13 Genotyping-by-Sequencing and Its Application to Oat Genomic Research
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    Chapter 14 Genome-Wide Association Analysis Using R
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    Chapter 15 De Novo Transcriptome Assembly in Polyploid Species
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    Chapter 16 Isolation of Oat (Avena sativa L.) Total Proteins and Their Prolamin Fractions for 2D Electrophoresis
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    Chapter 17 2-DE Separation and Identification of Oat (Avena sativa L.) Proteins and Their Prolamin Fractions
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    Chapter 18 Selected Bioinformatic Tools and MS (MALDI-TOF, PMF) Techniques Used in the Strategy for the Identification of Oat Proteins After 2-DE
Attention for Chapter 9: Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry
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Chapter title
Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry
Chapter number 9
Book title
Oat
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6682-0_9
Pubmed ID
Book ISBNs
978-1-4939-6680-6, 978-1-4939-6682-0
Authors

Veronica M. T. Lattanzio, Michelangelo Pascale

Editors

Sebastian Gasparis

Abstract

This protocol specifies an accurate and sensitive method for the determination of T-2 and HT-2 toxins content in oats and oat-based foods using liquid chromatography with tandem mass spectrometry detection. T-2 and HT-2 toxins are extracted from the test material with a mixture of acetonitrile and water. The filtered extract is dried, reconstituted with a mixture of methanol and water, then purified on a polymeric solid-phase extraction cartridge. Toxins are finally eluted from the column with methanol and quantified by reversed phase high performance liquid chromatography with tandem mass spectrometry detection.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 33%
Student > Doctoral Student 2 33%
Researcher 1 17%
Student > Ph. D. Student 1 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 50%
Biochemistry, Genetics and Molecular Biology 1 17%
Medicine and Dentistry 1 17%
Unknown 1 17%