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CD95

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Cover of 'CD95'

Table of Contents

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    Book Overview
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    Chapter 1 Production of the Non-apoptotic Metalloprotease-Cleaved CD95L and Its Cytotoxic Recombinant Counterpart Designed Ig-CD95L
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    Chapter 2 CD95 Stimulation with CD95L and DISC Analysis
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    Chapter 3 Immunoprecipitation of Death Inducing Signaling Complex by Caspase-8
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    Chapter 4 In Vitro Evaluation of the Apoptosis Function in Human Activated T Cells
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    Chapter 5 Proximity Ligation Assay (PLA) to Evaluate DISC and MISC Composition
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    Chapter 6 Fluorometric Methods for Detection of Mitochondrial Membrane Depolarization Induced by CD95 Activation
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    Chapter 7 Generation and Application of Bioluminescent CD95 Ligand Fusion Proteins
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    Chapter 8 CD95-Mediated Calcium Signaling
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    Chapter 9 CD95-Mediated Proton Regulation
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    Chapter 10 Study of the CD95-Mediated Non-apoptotic Signaling Pathway: PI3K
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    Chapter 11 Organelle Separation and Cell Signaling
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    Chapter 12 Boyden Chamber Assay to Study of Cell Migration Induced by Metalloprotease Cleaved-CD95L
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    Chapter 13 Isolation of Lipid Rafts Through Discontinuous Sucrose Gradient Centrifugation and Fas/CD95 Death Receptor Localization in Raft Fractions
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    Chapter 14 Quantifying CD95/cl-CD95L Implications in Cell Mechanics and Membrane Tension by Atomic Force Microscopy Based Force Measurements
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    Chapter 15 Sketching of CD95 Oligomers by In Silico Investigations
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    Chapter 16 Site-Specific Detection of Tyrosine Phosphorylated CD95 Following Protein Separation by Conventional and Phospho-Protein Affinity SDS-PAGE
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    Chapter 17 Detection of S-Acylated CD95 by Acyl-Biotin Exchange
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    Chapter 18 Exploration of Fas S-Nitrosylation by the Biotin Switch Assay
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    Chapter 19 Method to Measure Sphingomyelin Synthase Activity Changes in Response to CD95L
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    Chapter 20 Liquid Chromatography–High Resolution Mass Spectrometry Method to Study Sphingolipid Metabolism Changes in Response to CD95L
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    Chapter 21 CD95 and the MRL-lpr Mouse Model
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    Chapter 22 Erratum
Attention for Chapter 14: Quantifying CD95/cl-CD95L Implications in Cell Mechanics and Membrane Tension by Atomic Force Microscopy Based Force Measurements
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Chapter title
Quantifying CD95/cl-CD95L Implications in Cell Mechanics and Membrane Tension by Atomic Force Microscopy Based Force Measurements
Chapter number 14
Book title
CD95
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6780-3_14
Pubmed ID
Book ISBNs
978-1-4939-6778-0, 978-1-4939-6780-3, 978-1-4939-6778-0, 978-1-4939-6780-3
Authors

Anaïs Sadoun, Pierre-Henri Puech

Editors

Patrick Legembre

Abstract

Atomic force microscopy (AFM) is an invaluable tool to investigate the structure of biological material surfaces by imaging them at nanometer scale in physiological conditions. It can also be used to measure the forces and mechanics from single molecule interaction to cell-cell adhesion. Here, we present a methodology that allows to quantify cell elastic properties (using the Young modulus) and cell membrane tension modulated by CD95/cl-CD95L interactions by coupling nanoindentation and membrane tube pulling using suitably decorated AFM levers.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 25%
Student > Ph. D. Student 1 25%
Researcher 1 25%
Unknown 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 50%
Physics and Astronomy 1 25%
Unknown 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 June 2017.
All research outputs
#20,390,619
of 22,940,083 outputs
Outputs from Methods in molecular biology
#9,915
of 13,127 outputs
Outputs of similar age
#355,763
of 420,863 outputs
Outputs of similar age from Methods in molecular biology
#842
of 1,074 outputs
Altmetric has tracked 22,940,083 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,127 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 420,863 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.