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Chapter title |
CD95-Mediated Proton Regulation
|
---|---|
Chapter number | 9 |
Book title |
CD95
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6780-3_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6778-0, 978-1-4939-6780-3
|
Authors |
Auréa Cophignon, Mallorie Poët, Michael Monet, Michel Tauc, Laurent Counillon |
Editors |
Patrick Legembre |
Abstract |
The Na(+)/H(+) exchanger NHE1 is at the crossroads of a large diversity of signaling pathways, whose activation modifies the cooperative response of the transporter to intracellular H(+) ions. Here we show how the activation of the Na(+)/H(+) exchanger NHE1 by the cleaved ligand of CD95 can be measured. We demonstrate two different methods designed to set intracellular pH at precise values. Then we show how these can be coupled to fast kinetics of lithium transport, which will enable to measure the NHE1 activity like for an enzyme, because they will yield rates of transport. |