Chapter title |
Two-Dimensional Electrophoresis Protocols to Analyze the Microtubule-Associated Tau Proteins from Several Biological Sources.
|
---|---|
Chapter number | 14 |
Book title |
Tau Protein
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6598-4_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6596-0, 978-1-4939-6598-4
|
Authors |
Nicolas Sergeant, Francisco-Jose Fernandez-Gomez, Helene Obriot, Sabiha Eddarkaoui, Valérie Buée-Scherrer, Luc Buée, Sergeant, Nicolas, Fernandez-Gomez, Francisco-Jose, Obriot, Helene, Eddarkaoui, Sabiha, Buée-Scherrer, Valérie, Buée, Luc |
Editors |
Caroline Smet-Nocca |
Abstract |
Technology breakthrough in proteomics enables to gather qualitative and quantitative information about a protein or a complex mixture of proteins. Two-dimensional gel electrophoresis remains an interesting technique, which provides an overview of the complexity of isovariants from a single protein when coupled to western blotting. Here, we describe a detailed protocol for the two-dimensional analysis of microtubule-associated Tau isovariants from cell to human or mouse brain tissue. We provide protocol enabling to separate native and Tau proteins that are aggregated in neurodegenerative disorders such as Alzheimer's disease. |
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