Chapter title |
Detection and Quantification Methods for Fibrillar Products of In Vitro Tau Aggregation Assays.
|
---|---|
Chapter number | 6 |
Book title |
Tau Protein
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6598-4_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6596-0, 978-1-4939-6598-4
|
Authors |
Niki Nanavaty, Lauren Lin, Samantha H. Hinckley, Jeff Kuret Ph.D., Nanavaty, Niki, Lin, Lauren, Hinckley, Samantha H, Kuret, Jeff, Jeff Kuret |
Editors |
Caroline Smet-Nocca |
Abstract |
Alzheimer's disease is characterized in part by the intracellular misfolding and aggregation of tau protein. The aggregates, which range in size from small oligomers to large filaments, are markers for disease diagnosis and staging, potential vectors for disease propagation, and candidate sources of neurotoxicity. Here we present protocols for synthesizing large tau aggregates characterized by filamentous morphology and cross-β-sheet structure from monomeric full-length tau precursors in vitro. We also describe their detection and quantification through thioflavin dye binding, filter trap, and transmission electron microscopy methods. These methods cover applications requiring high-throughput capability as well as those requiring high-resolution analysis of aggregation mechanism. |
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