Chapter title |
The Study of Posttranslational Modifications of Tau Protein by Nuclear Magnetic Resonance Spectroscopy: Phosphorylation of Tau Protein by ERK2 Recombinant Kinase and Rat Brain Extract, and Acetylation by Recombinant Creb-Binding Protein.
|
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Chapter number | 11 |
Book title |
Tau Protein
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6598-4_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6596-0, 978-1-4939-6598-4
|
Authors |
Haoling Qi, Clément Despres, Sudhakaran Prabakaran, François-Xavier Cantrelle, Béatrice Chambraud, Jeremy Gunawardena, Guy Lippens, Caroline Smet-Nocca, Isabelle Landrieu, Qi, Haoling, Despres, Clément, Prabakaran, Sudhakaran, Cantrelle, François-Xavier, Chambraud, Béatrice, Gunawardena, Jeremy, Lippens, Guy, Smet-Nocca, Caroline, Landrieu, Isabelle |
Editors |
Caroline Smet-Nocca |
Abstract |
Nuclear magnetic resonance (NMR) spectroscopy can be used as an analytical tool to investigate posttranslational modifications of protein. NMR is a valuable tool to map the interaction regions of protein partners. Here, we present protocols that have been developed in the course of our studies of the neuronal Tau protein. Tau is found aggregated in the neurons of Alzheimer's disease patients. Development of the disease is accompanied by increased, abnormal phosphorylation and acetylation of Tau. We have used NMR to investigate how these posttranslational modifications of Tau affect the interactions with its partners. We present here detailed protocols of in vitro phosphorylation of Tau by recombinant kinase, ERK2, or kinase activity of rat brain extracts, and acetylation by recombinant Creb-binding protein (CBP) acetyltransferase. The analytical characterization of the modified Tau by NMR spectroscopy is additionally described. |
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Geographical breakdown
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Unknown | 42 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 7 | 17% |
Student > Bachelor | 6 | 14% |
Student > Master | 5 | 12% |
Researcher | 5 | 12% |
Other | 5 | 12% |
Unknown | 7 | 17% |
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Chemistry | 6 | 14% |
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Psychology | 2 | 5% |
Other | 2 | 5% |
Unknown | 14 | 33% |