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Multiplex Biomarker Techniques

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Cover of 'Multiplex Biomarker Techniques'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Application of Multiplex Biomarker Approaches to Accelerate Drug Discovery and Development.
  3. Altmetric Badge
    Chapter 2 The Application of Multiplex Biomarker Techniques for Improved Stratification and Treatment of Schizophrenia Patients.
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    Chapter 3 Multiplex Biomarker Approaches in Type 2 Diabetes Mellitus Research.
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    Chapter 4 LC-MS(E), Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics.
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    Chapter 5 Phenotyping Multiple Subsets of Immune Cells In Situ in FFPE Tissue Sections: An Overview of Methodologies.
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    Chapter 6 Identification and Clinical Translation of Biomarker Signatures: Statistical Considerations.
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    Chapter 7 Opportunities and Challenges of Multiplex Assays: A Machine Learning Perspective.
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    Chapter 8 Multiplex Analyses Using Real-Time Quantitative PCR.
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    Chapter 9 Multiplex Analysis Using cDNA Transcriptomic Profiling.
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    Chapter 10 Multiplex Single Nucleotide Polymorphism Analyses.
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    Chapter 11 Pulsed SILAC as a Approach for miRNA Targets Identification in Cell Culture.
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    Chapter 12 Blood Bio-Sampling Procedures for Multiplex Biomarkers Studies.
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    Chapter 13 Multiplex Immunoassay Profiling.
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    Chapter 14 Multiplex Sequential Immunoprecipitation of Insulin Secretory Granule Proteins from Radiolabeled Pancreatic Islets.
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    Chapter 15 Two Dimensional Gel Electrophoresis of Insulin Secretory Granule Proteins from Biosynthetically-Labeled Pancreatic Islets.
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    Chapter 16 Depletion of Highly Abundant Proteins of the Human Blood Plasma: Applications in Proteomics Studies of Psychiatric Disorders.
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    Chapter 17 Simultaneous Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Analysis of Two Distinct Proteomes.
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    Chapter 18 Selective Reaction Monitoring for Quantitation of Cellular Proteins.
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    Chapter 19 Characterization of a Protein Interactome by Co-Immunoprecipitation and Shotgun Mass Spectrometry.
  21. Altmetric Badge
    Chapter 20 Using (15)N-Metabolic Labeling for Quantitative Proteomic Analyses.
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    Chapter 21 Multiplex Measurement of Serum Folate Vitamers by UPLC-MS/MS.
  23. Altmetric Badge
    Chapter 22 UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life.
  24. Altmetric Badge
    Chapter 23 iTRAQ-Based Shotgun Proteomics Approach for Relative Protein Quantification.
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    Chapter 24 (1)H NMR Metabolomic Profiling of Human and Animal Blood Serum Samples.
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    Chapter 25 Lab-on-a-Chip Multiplex Assays.
  27. Altmetric Badge
    Chapter 26 Multiplex Smartphone Diagnostics.
  28. Altmetric Badge
    Chapter 27 Development of a User-Friendly App for Assisting Anticoagulation Treatment.
  29. Altmetric Badge
    Chapter 28 Multiplex Biomarker Approaches to Enable Point-of-Care Testing and Personalized Medicine.
Attention for Chapter 11: Pulsed SILAC as a Approach for miRNA Targets Identification in Cell Culture.
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Chapter title
Pulsed SILAC as a Approach for miRNA Targets Identification in Cell Culture.
Chapter number 11
Book title
Multiplex Biomarker Techniques
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6730-8_11
Pubmed ID
Book ISBNs
978-1-4939-6729-2, 978-1-4939-6730-8
Authors

Daniella E. Duque-Guimarães, Juliana de Almeida-Faria, Thomas Prates Ong, Susan E. Ozanne, Duque-Guimarães, Daniella E, de Almeida-Faria, Juliana, Ong, Thomas Prates, Ozanne, Susan E, Duque-Guimarães, Daniella E., Ozanne, Susan E., Almeida-Faria, Juliana

Editors

Paul C. Guest

Abstract

Pulsed stable isotope labeling by amino acids in cell culture (pSILAC) comprises a variation of the classical SILAC proteomic methodology that enables the identification of short-term proteomic responses such as those elicited by micro RNAs (miRNAs). Here, we describe a detailed pSILAC protocol for global identification and quantification of protein translation alterations induced by a miRNA using 3T3-L1 pre-adipocytes as a model system.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 14%
Professor > Associate Professor 1 14%
Student > Bachelor 1 14%
Student > Doctoral Student 1 14%
Unknown 3 43%
Readers by discipline Count As %
Business, Management and Accounting 1 14%
Computer Science 1 14%
Agricultural and Biological Sciences 1 14%
Medicine and Dentistry 1 14%
Unknown 3 43%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 December 2016.
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#18,487,595
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Outputs of similar age from Methods in molecular biology
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