Chapter title |
LC-MS(E), Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics.
|
---|---|
Chapter number | 4 |
Book title |
Multiplex Biomarker Techniques
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6730-8_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6729-2, 978-1-4939-6730-8
|
Authors |
Gustavo Henrique Martins Ferreira Souza, Paul C. Guest, Daniel Martins-de-Souza |
Editors |
Paul C. Guest |
Abstract |
Proteomic tools can only be implemented in clinical settings if high-throughput, automated, sensitive, and accurate methods are developed. This has driven researchers to the edge of mass spectrometry (MS)-based proteomics capacity. Here we provide an overview of recent achievements in mass spectrometric technologies and instruments. This includes development of high and ultra definition-MS(E) (HDMS(E) and UDMS(E)) through implementation of ion mobility (IM) MS towards sensitive and accurate label-free proteomics using ultra performance liquid chromatography (UPLC). Label free UPLC-HDMS(E) is less expensive than labeled-based quantitative proteomics and has no limits regarding the number of samples that can be analyzed and compared, which is an important requirement for supporting clinical applications. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 35 | 100% |
Demographic breakdown
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Researcher | 8 | 23% |
Student > Bachelor | 4 | 11% |
Student > Ph. D. Student | 3 | 9% |
Professor | 3 | 9% |
Other | 2 | 6% |
Other | 6 | 17% |
Unknown | 9 | 26% |
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Nursing and Health Professions | 1 | 3% |
Other | 6 | 17% |
Unknown | 15 | 43% |