| Chapter title |
Multiplex Analyses Using Real-Time Quantitative PCR.
|
|---|---|
| Chapter number | 8 |
| Book title |
Multiplex Biomarker Techniques
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6730-8_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6729-2, 978-1-4939-6730-8
|
| Authors |
Steve F. C. Hawkins, Paul C. Guest, Hawkins, Steve F. C., Guest, Paul C. |
| Editors |
Paul C. Guest |
| Abstract |
Quantitative polymerase chain reaction (qPCR) is a routinely used method for the detection and quantitation of gene expression in real time. Multiplex qPCR requires the use of probe-based assays, in which each probe is labeled with a unique fluorescent dye, resulting in different observed colors for each assay. The signal from each dye is used to quantitate the amount of each target separately in the same tube or well. The availability to multiplex therefore allows the measurement of the expression levels of several targets or genes of interest quickly. Here, we describe a method using the SensiFAST and SensiFAST One-Step probe kits which allows simultaneous real-time quantitation of up to 5 amplicons. |
Mendeley readers
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|---|---|---|
| Unknown | 179 | 100% |
Demographic breakdown
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|---|---|---|
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| Student > Ph. D. Student | 11 | 6% |
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| Unknown | 88 | 49% |
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| Unknown | 88 | 49% |