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Protein Arginylation

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Cover of 'Protein Arginylation'

Table of Contents

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    Book Overview
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    Chapter 1 Protein Arginylation: Milestones of Discovery
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    Chapter 2 Recollection of How We Came Across the Protein Modification with Amino Acids by Aminoacyl tRNA-Protein Transferase
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    Chapter 3 Arginyltransferase: A Personal and Historical Perspective
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    Chapter 4 Arginylation in a Partially Purified Fraction of 150 k xg Supernatants of Axoplasm and Injured Vertebrate Nerves.
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    Chapter 5 Preparation of ATE1 Enzyme from Native Mammalian Tissues
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    Chapter 6 Correlated Measurement of Endogenous ATE1 Activity on Native Acceptor Proteins in Tissues and Cultured Cells to Detect Cellular Aging
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    Chapter 7 Assaying the Posttranslational Arginylation of Proteins in Cultured Cells
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    Chapter 8 Assaying ATE1 Activity in Yeast by β-Gal Degradation
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    Chapter 9 Assaying Arginylation Activity in Cell Lysates Using a Fluorescent Reporter
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    Chapter 10 Assaying Intracellular Arginylation Activity Using a Fluorescent Reporter
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    Chapter 11 Bacterial Expression and Purification of Recombinant Arginyltransferase (ATE1) and Arg-tRNA Synthetase (RRS) for Arginylation Assays
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    Chapter 12 Preparation of tRNAArg for Arginylation Assay by In Vitro Transcription
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    Chapter 13 Preparation of an Enriched tRNAArg Fraction for Arginylation by Expression in E. coli
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    Chapter 14 Enzymatic Aminoacylation of tRNAArg Using Recombinant Arg-tRNA Synthetase
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    Chapter 15 Assaying ATE1 Activity In Vitro
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    Chapter 16 High-Throughput Arginylation Assay in Microplate Format
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    Chapter 17 Assaying for Arginyltransferase Activity and Specificity by Peptide Arrays
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    Chapter 18 Assay of Arginyltransferase Activity by a Fluorescent HPLC Method
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    Chapter 19 Identification of Arginylated Proteins by Mass Spectrometry
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    Chapter 20 Analysis of Arginylated Peptides by Subtractive Edman Degradation
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    Chapter 21 Transferase-Mediated Labeling of Protein N-Termini with Click Chemistry Handles
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    Chapter 22 Synthesis of Peptides and Proteins with Site-Specific Glutamate Arginylation
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    Chapter 23 Reconstitution of the Arginyltransferase (ATE1) Iron-Sulfur Cluster.
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    Chapter 24 N-Terminal Arginylation Pull-down Analysis Using the R-Catcher Tool
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    Chapter 25 Identification of Protein Arginylation by Encapsulated N-Terminal Peptide Enrichment Method
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    Chapter 26 Monitoring the Activation of Selective Autophagy via N-Terminal Arginylation
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    Chapter 27 Analyzing the Interaction of Arginylated Proteins and Nt-Arg-Mimicking Chemical Compounds to N-Recognins
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    Chapter 28 Synthesis of Stably Charged Arg-tRNAArg for Structural Analysis
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    Chapter 29 A High-Throughput Colorimetric Microplate Assay for Determination of Plasma Arginase Activity
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    Chapter 30 Development of New Tools for the Studies of Protein Arginylation
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Title
Protein Arginylation
Published by
Springer US, January 2023
DOI 10.1007/978-1-0716-2942-0
ISBNs
978-1-07-162941-3, 978-1-07-162942-0
Editors

Anna S. Kashina

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