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DNA Manipulation and Analysis

Overview of attention for book
Cover of 'DNA Manipulation and Analysis'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Classical Recombinant DNA Cloning
  3. Altmetric Badge
    Chapter 2 A Sequence- and Ligation-Independent Cloning (SLIC) Procedure for the Insertion of Genes into a Plasmid Vector
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    Chapter 3 Molecular Cloning Using In Vivo DNA Assembly
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    Chapter 4 Assembling Multiple Fragments: The Gibson Assembly
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    Chapter 5 TA Cloning Approaches to Cloning DNA with Damaged Ends DNA
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    Chapter 6 PCR-Based Assembly of Gene Sequences by Thermodynamically Balanced Inside-Out (TBIO) Gene Synthesis
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    Chapter 7 Random Mutagenesis by PCR
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    Chapter 8 In Vitro Site Directed Mutagenesis
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    Chapter 9 Xenopus Transgenesis Using the pGateway System
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    Chapter 10 CRISPR/Cas9 Gene Disruption Studies in F0 Xenopus Tadpoles: Understanding Development and Disease in the Frog
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    Chapter 11 A CRISPR/Cas-Based Method for Precise DNA Integration in Xenopus laevis Oocytes Followed by Intracytoplasmic Sperm Injection (ICSI) Fertilization
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    Chapter 12 A Lambda-Exonuclease SELEX Method for Generating Aptamers to Bacterial Targets
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    Chapter 13 Generation of Functional-RNA Arrays by In Vitro Transcription and In Situ RNA Capture for the Detection of RNA-RNA Interactions
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    Chapter 14 Chemical Synthesis of Oligonucelotide Sequences: Phosphoramidite Chemistry
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    Chapter 15 Low Throughput Direct Cycle Sequencing of Polymerase Chain Reaction (PCR) Products
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    Chapter 16 Nanopore Sequencing for Mixed Samples.
  18. Altmetric Badge
    Chapter 17 Ethics, Legality, and Safety for Geneticists
Attention for Chapter 16: Nanopore Sequencing for Mixed Samples.
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  • High Attention Score compared to outputs of the same age and source (93rd percentile)

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Chapter title
Nanopore Sequencing for Mixed Samples.
Chapter number 16
Book title
DNA Manipulation and Analysis
Published in
Methods in molecular biology, January 2023
DOI 10.1007/978-1-0716-3004-4_16
Pubmed ID
Book ISBNs
978-1-07-163003-7, 978-1-07-163004-4
Authors

Beckett, Angela H, Robson, Samuel C, Beckett, Angela H., Robson, Samuel C.

Abstract

Long read Nanopore sequencing can be utilised to determine the quality and accuracy of genetically engineered changes in animals, which often produce heterogenous samples. The protocol presented in this chapter can be used for a range of both low and high throughput sequencing applications. DNA must be repaired, barcoded and ligated to sequencing adapters prior to sequencing. Quality of sequencing data produced is dependent on stringent adherence to the protocol. However, nanopore sequencing is a fast moving field, therefore it is worth considering using the most up to date chemistry available.

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Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 March 2023.
All research outputs
#6,347,471
of 23,972,543 outputs
Outputs from Methods in molecular biology
#1,867
of 13,505 outputs
Outputs of similar age
#112,551
of 451,775 outputs
Outputs of similar age from Methods in molecular biology
#41
of 598 outputs
Altmetric has tracked 23,972,543 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 13,505 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 451,775 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 598 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.