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DNA Manipulation and Analysis

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Cover of 'DNA Manipulation and Analysis'

Table of Contents

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    Book Overview
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    Chapter 1 Classical Recombinant DNA Cloning
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    Chapter 2 A Sequence- and Ligation-Independent Cloning (SLIC) Procedure for the Insertion of Genes into a Plasmid Vector
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    Chapter 3 Molecular Cloning Using In Vivo DNA Assembly
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    Chapter 4 Assembling Multiple Fragments: The Gibson Assembly
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    Chapter 5 TA Cloning Approaches to Cloning DNA with Damaged Ends DNA
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    Chapter 6 PCR-Based Assembly of Gene Sequences by Thermodynamically Balanced Inside-Out (TBIO) Gene Synthesis
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    Chapter 7 Random Mutagenesis by PCR
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    Chapter 8 In Vitro Site Directed Mutagenesis
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    Chapter 9 Xenopus Transgenesis Using the pGateway System
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    Chapter 10 CRISPR/Cas9 Gene Disruption Studies in F0 Xenopus Tadpoles: Understanding Development and Disease in the Frog
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    Chapter 11 A CRISPR/Cas-Based Method for Precise DNA Integration in Xenopus laevis Oocytes Followed by Intracytoplasmic Sperm Injection (ICSI) Fertilization
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    Chapter 12 A Lambda-Exonuclease SELEX Method for Generating Aptamers to Bacterial Targets
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    Chapter 13 Generation of Functional-RNA Arrays by In Vitro Transcription and In Situ RNA Capture for the Detection of RNA-RNA Interactions
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    Chapter 14 Chemical Synthesis of Oligonucelotide Sequences: Phosphoramidite Chemistry
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    Chapter 15 Low Throughput Direct Cycle Sequencing of Polymerase Chain Reaction (PCR) Products
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    Chapter 16 Nanopore Sequencing for Mixed Samples.
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    Chapter 17 Ethics, Legality, and Safety for Geneticists
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Title
DNA Manipulation and Analysis
Published by
Springer US, March 2023
DOI 10.1007/978-1-0716-3004-4
ISBNs
978-1-07-163003-7, 978-1-07-163004-4
Editors

Scarlett, Garry

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