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Mitochondrial DNA

Overview of attention for book
Cover of 'Mitochondrial DNA'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Isolation of Functional Mitochondria and Pure mtDNA from Murine Tissues
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    Chapter 2 Isolating Mitochondria, Mitoplasts, and mtDNA from Cultured Mammalian Cells.
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    Chapter 3 Coupling Differential Centrifugation with Exonuclease Treatment and Size Exclusion Chromatography (DIFSEC) for Purification of mtDNA from Mammalian Cells
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    Chapter 4 Isolation and Quality Control of Yeast Mitochondria
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    Chapter 5 Mitochondrial DNA Isolation from Plants.
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    Chapter 6 Visualize the Distribution and Dynamics of Mitochondrial DNA (mtDNA) Nucleoids with Multiple Labeling Strategies
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    Chapter 7 Visualization of mtDNA Using FISH
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    Chapter 8 In Situ Analysis of Mitochondrial DNA Synthesis Using Metabolic Labeling Coupled to Fluorescence Microscopy.
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    Chapter 9 Measurement of Nucleoid Size Using STED Microscopy.
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    Chapter 10 How to Quantify DNA Compaction by TFAM with Acoustic Force Spectroscopy and Total Internal Reflection Fluorescence Microscopy
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    Chapter 11 Assessing TFAM Binding to Human Mitochondrial DNA
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    Chapter 12 Identification of Proximity Interactors of Mammalian Nucleoid Proteins by BioID
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    Chapter 13 Localization of Mitochondrial Nucleoids by Transmission Electron Microscopy Using the Transgenic Expression of the Mitochondrial Helicase Twinkle and APEX2
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    Chapter 14 In Vitro Assays of TWINKLE Function
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    Chapter 15 Rolling Circle Replication and Bypass of Damaged Nucleotides
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    Chapter 16 Studying Mitochondrial Nucleic Acid Synthesis Utilizing Intact Isolated Mitochondria
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    Chapter 17 Functional Assessment of Mitochondrial DNA Maintenance by Depletion and Repopulation Using 2’,3’-Dideoxycytidine in Cultured Cells
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    Chapter 18 Analysis of Mitochondrial DNA Replication by Two-Dimensional Agarose Gel Electrophoresis
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    Chapter 19 Quantitative Analysis of Nucleoside Triphosphate Pools in Mouse Muscle Using Hydrophilic Interaction Liquid Chromatography Coupled with Tandem Mass Spectrometry Detection
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    Chapter 20 Detection of UV-Induced Deletions in Mitochondrial DNA
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    Chapter 21 Determination of the Ribonucleotide Content of mtDNA Using Alkaline Gels
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    Chapter 22 5′-End Mapping in Human Mitochondrial DNA
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    Chapter 23 Manipulation of Murine Mitochondrial DNA Heteroplasmy with mtZFNs
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    Chapter 24 Biolistic Transformation of Chlamydomonas reinhardtii and Saccharomyces cerevisiae Mitochondria
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    Chapter 25 A Method for Precisely Identifying Modifications to Plant Mitochondrial Genomes by mitoTALENs.
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    Chapter 26 Mitochondrial DNA Sequencing and Heteroplasmy Quantification by Next Generation Sequencing
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    Chapter 27 Genomic Strategies in Mitochondrial Diagnostics.
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    Chapter 28 Mitochondrial DNA Enrichment for Sensitive Next-Generation Sequencing.
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    Chapter 29 Single Cell Analysis of Mitochondrial DNA Deletions
Attention for Chapter 2: Isolating Mitochondria, Mitoplasts, and mtDNA from Cultured Mammalian Cells.
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Chapter title
Isolating Mitochondria, Mitoplasts, and mtDNA from Cultured Mammalian Cells.
Chapter number 2
Book title
Mitochondrial DNA
Published in
Methods in molecular biology, January 2023
DOI 10.1007/978-1-0716-2922-2_2
Pubmed ID
36807781
Book ISBNs
978-1-07-162921-5, 978-1-07-162922-2
Authors

Menger, Katja E, Nicholls, Thomas J, Menger, Katja E., Nicholls, Thomas J.

Abstract

Mitochondria are double membrane-bound eukaryotic organelles with roles in a range of cellular activities including energy conversion, apoptosis, cell signalling, and the biosynthesis of enzyme cofactors. Mitochondria contain their own genome, called mtDNA, which encodes subunits of the oxidative phosphorylation machinery as well as the rRNA and tRNA molecules required for their translation within mitochondria. The ability to isolate highly purified mitochondria from cells has been instrumental in a number of studies of mitochondrial function. Differential centrifugation is a long-established method for the isolation of mitochondria. Cells are subjected to osmotic swelling and disruption, followed by centrifugation in isotonic sucrose solutions to separate mitochondria from other cellular components. We present a method using this principle for the isolation of mitochondria from cultured mammalian cell lines. Mitochondria purified by this method can be further fractionated to investigate protein localization, or act as a starting point to purify mtDNA.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 25%
Researcher 1 25%
Student > Doctoral Student 1 25%
Unknown 1 25%
Readers by discipline Count As %
Environmental Science 1 25%
Biochemistry, Genetics and Molecular Biology 1 25%
Agricultural and Biological Sciences 1 25%
Unknown 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 February 2023.
All research outputs
#17,275,842
of 25,378,284 outputs
Outputs from Methods in molecular biology
#5,988
of 14,183 outputs
Outputs of similar age
#270,819
of 479,477 outputs
Outputs of similar age from Methods in molecular biology
#287
of 722 outputs
Altmetric has tracked 25,378,284 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 14,183 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
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We're also able to compare this research output to 722 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.

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