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Mitochondrial DNA

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Cover of 'Mitochondrial DNA'

Table of Contents

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    Book Overview
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    Chapter 1 Isolation of Functional Mitochondria and Pure mtDNA from Murine Tissues
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    Chapter 2 Isolating Mitochondria, Mitoplasts, and mtDNA from Cultured Mammalian Cells.
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    Chapter 3 Coupling Differential Centrifugation with Exonuclease Treatment and Size Exclusion Chromatography (DIFSEC) for Purification of mtDNA from Mammalian Cells
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    Chapter 4 Isolation and Quality Control of Yeast Mitochondria
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    Chapter 5 Mitochondrial DNA Isolation from Plants.
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    Chapter 6 Visualize the Distribution and Dynamics of Mitochondrial DNA (mtDNA) Nucleoids with Multiple Labeling Strategies
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    Chapter 7 Visualization of mtDNA Using FISH
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    Chapter 8 In Situ Analysis of Mitochondrial DNA Synthesis Using Metabolic Labeling Coupled to Fluorescence Microscopy.
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    Chapter 9 Measurement of Nucleoid Size Using STED Microscopy.
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    Chapter 10 How to Quantify DNA Compaction by TFAM with Acoustic Force Spectroscopy and Total Internal Reflection Fluorescence Microscopy
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    Chapter 11 Assessing TFAM Binding to Human Mitochondrial DNA
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    Chapter 12 Identification of Proximity Interactors of Mammalian Nucleoid Proteins by BioID
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    Chapter 13 Localization of Mitochondrial Nucleoids by Transmission Electron Microscopy Using the Transgenic Expression of the Mitochondrial Helicase Twinkle and APEX2
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    Chapter 14 In Vitro Assays of TWINKLE Function
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    Chapter 15 Rolling Circle Replication and Bypass of Damaged Nucleotides
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    Chapter 16 Studying Mitochondrial Nucleic Acid Synthesis Utilizing Intact Isolated Mitochondria
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    Chapter 17 Functional Assessment of Mitochondrial DNA Maintenance by Depletion and Repopulation Using 2’,3’-Dideoxycytidine in Cultured Cells
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    Chapter 18 Analysis of Mitochondrial DNA Replication by Two-Dimensional Agarose Gel Electrophoresis
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    Chapter 19 Quantitative Analysis of Nucleoside Triphosphate Pools in Mouse Muscle Using Hydrophilic Interaction Liquid Chromatography Coupled with Tandem Mass Spectrometry Detection
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    Chapter 20 Detection of UV-Induced Deletions in Mitochondrial DNA
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    Chapter 21 Determination of the Ribonucleotide Content of mtDNA Using Alkaline Gels
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    Chapter 22 5′-End Mapping in Human Mitochondrial DNA
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    Chapter 23 Manipulation of Murine Mitochondrial DNA Heteroplasmy with mtZFNs
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    Chapter 24 Biolistic Transformation of Chlamydomonas reinhardtii and Saccharomyces cerevisiae Mitochondria
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    Chapter 25 A Method for Precisely Identifying Modifications to Plant Mitochondrial Genomes by mitoTALENs.
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    Chapter 26 Mitochondrial DNA Sequencing and Heteroplasmy Quantification by Next Generation Sequencing
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    Chapter 27 Genomic Strategies in Mitochondrial Diagnostics.
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    Chapter 28 Mitochondrial DNA Enrichment for Sensitive Next-Generation Sequencing.
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    Chapter 29 Single Cell Analysis of Mitochondrial DNA Deletions
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Title
Mitochondrial DNA
Published by
Springer US, March 2023
DOI 10.1007/978-1-0716-2922-2
ISBNs
978-1-07-162921-5, 978-1-07-162922-2
Editors

Nicholls, Thomas J., Uhler, Jay P., Falkenberg, Maria

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