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Genome Editing in Animals

Overview of attention for book
Cover of 'Genome Editing in Animals'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Construction and Evaluation of Zinc Finger Nucleases
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    Chapter 2 Updated Overview of TALEN Construction Systems
  4. Altmetric Badge
    Chapter 3 CRISPR/Cas9
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    Chapter 4 SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gene Expression by CRISPR System.
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    Chapter 5 Utilizing Large Functional and Population Genomics Resources for CRISPR/Cas Perturbation Experiment Design.
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    Chapter 6 Generation of Genome-Edited Mice by Cytoplasmic Injection of CRISPR-Cas9 RNA
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    Chapter 7 Gene Targeting in Mouse Embryonic Stem Cells via CRISPR/Cas9 Ribonucleoprotein (RNP)-Mediated Genome Editing
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    Chapter 8 Generation of Knock-In Mouse by Genome Editing
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    Chapter 9 Introduction of Genetic Mutations Into Mice by Base Editor and Target-AID
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    Chapter 10 Genome Editing in Mouse and Rat by Electroporation.
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    Chapter 11 Generation of Floxed Mice by Sequential Electroporation
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    Chapter 12 Efficient Detection of Flox Mice Using In Vitro Cre Recombination
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    Chapter 13 VCre/VloxP and SCre/SloxP as Reliable Site-Specific Recombination Systems for Genome Engineering
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    Chapter 14 Efficient CRISPR/Cas9-Assisted Knockin of Large DNA Donors by Pronuclear Microinjection During S-Phase in Mouse Zygotes
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    Chapter 15 Genome Editing of Murine Liver Hepatocytes by AAV Vector-Mediated Expression of Cas9 In Vivo.
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    Chapter 16 Nonviral Ex Vivo Genome Editing in Mouse Bona Fide Hematopoietic Stem Cells with CRISPR/Cas9.
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    Chapter 17 Genome Editing of Rat.
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    Chapter 18 A Simple and Efficient Method for Generating KO Rats Using In Vitro Fertilized Oocytes
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    Chapter 19 Editing the Genome of the Golden Hamster (Mesocricetus auratus)
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    Chapter 20 Gene Targeting in Rabbits: Single-Step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids
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    Chapter 21 Genome Editing of Pig
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    Chapter 22 GEEP Method: An Optimized Electroporation-Mediated Gene Editing Approach for Establishment of Knockout Pig Lines
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    Chapter 23 Genome Editing Mediated by Primordial Germ Cell in Chicken.
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    Chapter 24 CRISPR-Cas9-Mediated Genome Modifications in Zebrafish.
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    Chapter 25 Genome Editing of Medaka
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    Chapter 26 CRISPR-Cas9-Based Functional Analysis in Amphibians: Xenopus laevis, Xenopus tropicalis, and Pleurodeles waltl.
  28. Altmetric Badge
    Chapter 27 Genome Editing of Silkworms
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    Chapter 28 Improved Genome Editing in the Ascidian Ciona with CRISPR/Cas9 and TALEN
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    Chapter 29 Genome Editing of C. elegans.
Attention for Chapter 24: CRISPR-Cas9-Mediated Genome Modifications in Zebrafish.
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Chapter title
CRISPR-Cas9-Mediated Genome Modifications in Zebrafish.
Chapter number 24
Book title
Genome Editing in Animals
Published in
Methods in molecular biology, January 2023
DOI 10.1007/978-1-0716-3016-7_24
Pubmed ID
36773157
Book ISBNs
978-1-07-163015-0, 978-1-07-163016-7
Authors

Kamachi, Yusuke, Kawahara, Atsuo

Abstract

CRISPR-Cas9 genome editing technology has been successfully applied to generate various genetic modifications in zebrafish. The CRISPR-Cas9 system, which originally consisted of three components, CRISPR RNA (crRNA), trans-activating crRNA (tracrRNA), and Cas9, efficiently induces DNA double-strand breaks (DSBs) at targeted genomic loci, often resulting in frameshift-mediated target gene disruption (knockout). However, it remains difficult to perform the targeted integration of exogenous DNA fragments (knock-in) with CRISPR-Cas9. DSBs can be restored through DNA repair mechanisms, such as nonhomologous end joining (NHEJ), microhomology-mediated end joining (MMEJ), and homology-directed repair (HDR). One of our two research groups established a method for the precise MMEJ-mediated targeted integrations of exogenous genes containing homologous microhomology sequences flanking a targeted genomic locus in zebrafish. The other group recently developed a method for knocking in ~200 nt sequences encoding composite tags using long single-stranded DNA (ssDNA) donors. This chapter summarizes the CRISPR-Cas9-mediated genome modification strategy in zebrafish.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 50%
Student > Master 1 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 February 2023.
All research outputs
#15,706,682
of 23,341,064 outputs
Outputs from Methods in molecular biology
#5,497
of 13,337 outputs
Outputs of similar age
#226,122
of 427,261 outputs
Outputs of similar age from Methods in molecular biology
#161
of 391 outputs
Altmetric has tracked 23,341,064 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,337 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 427,261 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 391 others from the same source and published within six weeks on either side of this one. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.

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