Chapter title |
Studying Whole-Genome Duplication Using Experimental Evolution of Spirodela polyrhiza.
|
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Chapter number | 19 |
Book title |
Polyploidy
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Published in |
Methods in molecular biology, January 2023
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DOI | 10.1007/978-1-0716-2561-3_19 |
Pubmed ID | |
Book ISBNs |
978-1-07-162560-6, 978-1-07-162561-3
|
Authors |
Wu, Tian, Natran, Annelore, Prost, Lucas, Aydogdu, Eylem, Van de Peer, Yves, Bafort, Quinten, Aydogdu Lohaus, Eylem, Tian Wu, Annelore Natran, Lucas Prost, Eylem Aydogdu, Yves Van de Peer, Quinten Bafort |
Abstract |
In this chapter, we present the use of Spirodela polyrhiza in experiments designed to study the evolutionary impact of whole-genome duplication (WGD). We shortly introduce this duckweed species and explain why it is a suitable model for experimental evolution. Subsequently, we discuss the most relevant steps and methods in the design of a ploidy-related duckweed experiment. These steps include strain selection, ploidy determination, different methods of making polyploid duckweeds, replication, culturing conditions, preservation, and the ways to quantify phenotypic and transcriptomic change. |
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Geographical breakdown
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Belgium | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |