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Polyploidy

Overview of attention for book
Cover of 'Polyploidy'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Inference of Ancient Polyploidy from Genomic Data
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    Chapter 2 Navigating the CoGe Online Software Suite for Polyploidy Research
  4. Altmetric Badge
    Chapter 3 Inference of Ancient Polyploidy Using Transcriptome Data.
  5. Altmetric Badge
    Chapter 4 POInT: Modeling Polyploidy in the Era of Ubiquitous Genomics
  6. Altmetric Badge
    Chapter 5 Applying Machine Learning to Classify the Origins of Gene Duplications
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    Chapter 6 Phasing Gene Copies into Polyploid Subgenomes Using a Bayesian Phylogenetic Approach
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    Chapter 7 Constraining Whole-Genome Duplication Events in Geological Time
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    Chapter 8 SCORPiOs, a Novel Method to Reconstruct Gene Phylogenies in the Context of a Known WGD Event
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    Chapter 9 Inferring Chromosome Number Changes Along a Phylogeny Using chromEvol
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    Chapter 10 PURC Provides Improved Sequence Inference for Polyploid Phylogenetics and Other Manifestations of the Multiple-Copy Problem.
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    Chapter 11 Analyses of Genome Regulatory Evolution Following Whole-Genome Duplication Using the Phylogenetic EVE Model
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    Chapter 12 Beyond Transcript Concentrations: Quantifying Polyploid Expression Responses per Biomass, per Genome, and per Cell with RNA-Seq.
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    Chapter 13 A Robust Methodology for Assessing Homoeolog-Specific Expression
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    Chapter 14 Analyzing Autopolyploid Genetic Data Using GenoDive
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    Chapter 15 Inference of Polyploid Origin and Inheritance Mode from Population Genomic Data
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    Chapter 16 Population Genomic Analysis of Diploid-Autopolyploid Species.
  18. Altmetric Badge
    Chapter 17 Inferring the Demographic History and Inheritance Mode of Tetraploid Species Using ABC
  19. Altmetric Badge
    Chapter 18 Studying Whole-Genome Duplication Using Experimental Evolution of Chlamydomonas
  20. Altmetric Badge
    Chapter 19 Studying Whole-Genome Duplication Using Experimental Evolution of Spirodela polyrhiza.
  21. Altmetric Badge
    Chapter 20 Experimental Approaches to Generate and Isolate Human Tetraploid Cells
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    Chapter 21 Measuring Cellular Ploidy In Situ by Light Microscopy.
  23. Altmetric Badge
    Chapter 22 Using Mosaic Cell Labeling to Visualize Polyploid Cells in the Drosophila Brain
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    Chapter 23 Sequencing and Assembly of Polyploid Genomes.
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    Chapter 24 Genome Editing by CRISPR/Cas9 in Polyploids
  26. Altmetric Badge
    Chapter 25 Developing a CRISPR System in Nongenetic Model Polyploids.
  27. Altmetric Badge
    Chapter 26 Efficiently Editing Multiple Duplicated Homeologs and Alleles for Recurrent Polyploids.
Attention for Chapter 21: Measuring Cellular Ploidy In Situ by Light Microscopy.
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)
  • High Attention Score compared to outputs of the same age and source (97th percentile)

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Chapter title
Measuring Cellular Ploidy In Situ by Light Microscopy.
Chapter number 21
Book title
Polyploidy
Published in
Methods in molecular biology, January 2023
DOI 10.1007/978-1-0716-2561-3_21
Pubmed ID
36720825
Book ISBNs
978-1-07-162560-6, 978-1-07-162561-3
Authors

Clay, Delisa E, Stormo, Benjamin M, Fox, Donald T, Clay, Delisa E., Stormo, Benjamin M., Fox, Donald T.

Abstract

Determining cellular DNA content is valuable in the study of numerous biological processes, including organ development and injury repair. While FACS analysis of dissociated cells is a widely used method for assaying ploidy in a tissue cell population, for many tissue samples, it is possible and convenient to measure ploidy in situ using light microscopy. Here, we present two protocols for measuring cellular ploidy in tissues. These protocols are based on our studies in Drosophila melanogaster, but these are applicable to other settings as well. We present example results from Drosophila hindgut, midgut, and wing imaginal disc as examples. The first protocol focuses on measuring DNA content from decondensed interphase nuclei, while the second protocol details the visualization of condensed chromosomes for ploidy determination, either from mitotic cells or from interphase cells with drug-induced chromosome condensation. These techniques can be completed in 1 day and require standard lab supplies as well as a fluorescence light microscope.

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 Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2023.
All research outputs
#3,429,972
of 25,540,105 outputs
Outputs from Methods in molecular biology
#698
of 14,262 outputs
Outputs of similar age
#70,045
of 477,385 outputs
Outputs of similar age from Methods in molecular biology
#20
of 715 outputs
Altmetric has tracked 25,540,105 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 14,262 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done particularly well, scoring higher than 95% of its peers.
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We're also able to compare this research output to 715 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.

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