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Antibiotics

Overview of attention for book
Cover of 'Antibiotics'

Table of Contents

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    Book Overview
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    Chapter 1 Antibiotics: Precious Goods in Changing Times
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    Chapter 2 A Whole-Cell Assay for Detection of Antibacterial Activity in Actinomycete Culture Supernatants
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    Chapter 3 Sampling of Human Microbiomes to Screen for Antibiotic-Producing Commensals
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    Chapter 4 Production of Antimicrobial Compounds by Homologous and Heterologous Expression
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    Chapter 5 Isolation and Purification of Natural Products from Microbial Cultures
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    Chapter 6 Structure Elucidation of Antibiotics
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    Chapter 7 Computer-Aided Drug Design: An Update
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    Chapter 8 Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents
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    Chapter 9 Microscopy-Based Multiwell Assay to Characterize Disturbed Bacterial Morphogenesis Upon Antibiotic Action
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    Chapter 10 Expansion Microscopy of Bacillus subtilis
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    Chapter 11 Tracking Global and Local Changes in Membrane Fluidity Through Fluorescence Spectroscopy and Microscopy
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    Chapter 12 Quantitative Analysis of Microscopy Data to Evaluate Bacterial Responses to Antibiotic Treatment
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    Chapter 13 Application of a Bacillus subtilis Whole-Cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds
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    Chapter 14 Determination of Bacterial Membrane Impairment by Antimicrobial Agents
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    Chapter 15 A Colorimetric Assay to Identify and Characterize Bacterial Primase Inhibitors.
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    Chapter 16 Cell-Based Fluorescent Screen Amenable to HTS to Identify Inhibitors of Bacterial Translation Initiation
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    Chapter 17 Bacterial Two Component Systems: Overexpression and Purification: In Vitro and In Vivo Inhibitor Screens
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    Chapter 18 Sample Preparation for Mass Spectrometry-Based Absolute Quantification of Bacterial Proteins in Antibiotic Stress Research
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    Chapter 19 Elemental Analysis for the Characterization of Antimicrobial Effects
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    Chapter 20 Label-Free Quantitation of Ribosomal Proteins from Bacillus subtilis for Antibiotic Research
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    Chapter 21 Functional Metagenomics to Study Antibiotic Resistance.
Attention for Chapter 15: A Colorimetric Assay to Identify and Characterize Bacterial Primase Inhibitors.
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Chapter title
A Colorimetric Assay to Identify and Characterize Bacterial Primase Inhibitors.
Chapter number 15
Book title
Antibiotics
Published in
Methods in molecular biology, January 2023
DOI 10.1007/978-1-0716-2855-3_15
Pubmed ID
36445590
Book ISBNs
978-1-07-162854-6, 978-1-07-162855-3
Authors

Pang, Allan H, Tsodikov, Oleg V, Pang, Allan H., Tsodikov, Oleg V.

Abstract

Bacterial DNA primase DnaG is an attractive target for antibiotic discovery since it plays an essential role in DNA replication. Over the last 10 years, we have developed and optimized a robust colorimetric assay that enabled us to identify and validate inhibitors of bacterial primases. Here, we provide a detailed protocol for this colorimetric assay for DnaG from three different pathogenic bacteria (Mycobacterium tuberculosis, Bacillus anthracis, and Staphylococcus aureus), which can be performed in high throughput. We also describe secondary assays to characterize hits from this high-throughput screening assay. These assays are designed to identify inhibitors of the coupled enzyme inorganic pyrophosphatase, DNA binding agents, and elucidate the mode of inhibition of primase inhibitors.

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 Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 November 2022.
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#23,117,049
of 25,766,791 outputs
Outputs from Methods in molecular biology
#11,159
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#411,101
of 480,211 outputs
Outputs of similar age from Methods in molecular biology
#619
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