Chapter title |
Mass Spectrometry Multiplexed Detection of SARS-CoV-2.
|
---|---|
Chapter number | 12 |
Book title |
Multiplex Biomarker Techniques
|
Published in |
Methods in molecular biology, January 2022
|
DOI | 10.1007/978-1-0716-2395-4_12 |
Pubmed ID | |
Book ISBNs |
978-1-07-162394-7, 978-1-07-162395-4
|
Authors |
Viana, Luciana Godoy, Lebkuchen, Adriana, Schuch, Rodrigo Andrade, Okai, Guilherme Gonçalves, Salgueiro, Jessica Silva, Cardozo, Karina Helena Morais, Carvalho, Valdemir Melechco |
Abstract |
Testing of large populations for virus infection is now a reality worldwide due to the coronavirus (SARS-CoV-2) pandemic. The demand for SARS-CoV-2 testing using alternatives other than PCR led to the development of mass spectrometry (MS)-based assays. However, MS for SARS-CoV-2 large-scale testing have some downsides, including complex sample preparation and slow data analysis. Here, we describe a high-throughput targeted proteomics method to detect SARS-CoV-2 directly from nasopharyngeal and oropharyngeal swabs. This strategy employs fully automated sample preparation mediated by magnetic particles, followed by detection of SARS-CoV-2 nucleoprotein peptides by turbulent flow chromatography coupled with tandem mass spectrometry. |
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