Chapter title |
Liquid Chromatography-Mass Spectrometry Analysis of Peripheral Blood Mononuclear Cells from SARS-CoV-2 Infected Patients.
|
---|---|
Chapter number | 15 |
Book title |
Multiplex Biomarker Techniques
|
Published in |
Methods in molecular biology, January 2022
|
DOI | 10.1007/978-1-0716-2395-4_15 |
Pubmed ID | |
Book ISBNs |
978-1-07-162394-7, 978-1-07-162395-4
|
Authors |
Guest, Paul C, Rahmoune, Hassan, Guest, Paul C. |
Abstract |
COVID-19 disease is caused by infection with the SARS-CoV-2 virus and is associated with a cytokine storm effect in some patients. This can lead to decreased ability of the host to cope with the infection and result in severe disease outcomes. Here, we present a protocol for isolation of peripheral blood mononuclear cells (PBMCs) from COVID-19 patients followed by liquid chromatography-mass spectrometry (LC-MS) profiling to identify the affected molecules and molecular pathways. It is hoped that this will lead to the identification of potential biomarkers for monitoring the disease as well as treatment responses. This approach could also be used in the study of other respiratory viruses. |
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