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X Demographics
Mendeley readers
Attention Score in Context
Chapter title |
Accessing the Human Pluripotent Stem Cell Translatome by Polysome Profiling.
|
---|---|
Chapter number | 437 |
Book title |
Embryonic Stem Cell Protocols
|
Published in |
Methods in molecular biology, October 2021
|
DOI | 10.1007/7651_2021_437 |
Pubmed ID | |
Book ISBNs |
978-1-07-162436-4, 978-1-07-162437-1
|
Authors |
Gomes-Júnior, Rubens, Shigunov, Patrícia, Dallagiovanna, Bruno, Pereira, Isabela Tiemy |
Abstract |
Polysome profiling is a technique that uses sucrose density gradient ultracentrifugation to separate complexes of mRNAs associated with one or more ribosomes. Here we describe polysome profiling analysis in human pluripotent stem cells (hPSCs) using a continuous ultraviolet spectrophotometer and a gradient fractionator. We provide protocols for processing sucrose gradient fractions for isolation of RNA for RT-qPCR or large-scale sequencing analysis, used to establish the translational status of specific mRNAs and identify the role of noncoding RNA in translation. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 2 | 22% |
Student > Bachelor | 1 | 11% |
Other | 1 | 11% |
Researcher | 1 | 11% |
Unknown | 4 | 44% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Computer Science | 1 | 11% |
Engineering | 1 | 11% |
Unknown | 5 | 56% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 December 2021.
All research outputs
#15,686,478
of 23,310,485 outputs
Outputs from Methods in molecular biology
#5,493
of 13,324 outputs
Outputs of similar age
#247,993
of 434,153 outputs
Outputs of similar age from Methods in molecular biology
#90
of 274 outputs
Altmetric has tracked 23,310,485 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,324 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 434,153 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 274 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.