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Embryonic Stem Cell Protocols

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Cover of 'Embryonic Stem Cell Protocols'

Table of Contents

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    Book Overview
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    Chapter 400 ChIP-qPCR for Polycomb Group Proteins During Neuronal Differentiation of Human Pluripotent Stem Cells
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    Chapter 402 Feeder-Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol
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    Chapter 403 Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells
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    Chapter 404 Feeder-Free Human Embryonic Stem Cell Culture Under Defined Culture Conditions.
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    Chapter 405 High Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model
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    Chapter 406 Rat-Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish
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    Chapter 433 Embryoid Body Formation from Mouse and Human Pluripotent Stem Cells for Transplantation to Study Brain Microenvironment and Cellular Differentiation
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    Chapter 434 A Simple, Rapid, and Cost-Effective Method for Loss-of-Function Assays in Pluripotent Cells
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    Chapter 435 A Modified SMART-Seq Method for Single-Cell Transcriptomic Analysis of Embryoid Body Differentiation.
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    Chapter 436 Osteogenic Differentiation from Mouse Embryonic Stem Cells
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    Chapter 437 Accessing the Human Pluripotent Stem Cell Translatome by Polysome Profiling.
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    Chapter 438 Mouse Embryonic Stem Cell Culture in Serum-Containing or 2i Conditions
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    Chapter 439 Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages
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    Chapter 440 Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions.
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    Chapter 447 Murine Embryonic Stem Cell Culture, Self-Renewal, and Differentiation
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    Chapter 448 Directed Differentiation of Human Pluripotent Stem Cells into Inner Ear Organoids
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    Chapter 449 Glycolytic Profiling of Mouse Embryonic Stem Cells (mESCs)
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    Chapter 450 Replating Protocol for Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes
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    Chapter 459 Hematopoietic Cell Isolation by Antibody-Free Flow Cytometry in the Zebrafish Embryo
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    Chapter 460 Human Trophectoderm Spheroid Derived from Human Embryonic Stem Cells
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    Chapter 462 Proteomic Analysis of Human Neural Stem Cell Differentiation by SWATH-MS
Attention for Chapter 440: Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions.
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Chapter title
Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions.
Chapter number 440
Book title
Embryonic Stem Cell Protocols
Published in
Methods in molecular biology, October 2021
DOI 10.1007/7651_2021_440
Pubmed ID
Book ISBNs
978-1-07-162436-4, 978-1-07-162437-1
Authors

Isaja, Luciana, Ferriol-Laffouillere, Sofía Luján, Mucci, Sofía, Rodríguez-Varela, María Soledad, Romorini, Leonardo

Abstract

Human embryonic stem cells (hESCs) can differentiate into any cell lineage (pluripotency potential) derived from the three germ layers: ectoderm, mesoderm, and endoderm. Pluripotency is usually demonstrated in vitro by spontaneous differentiation of hESCs grown on a monolayer of feeder-cells using an embryoid bodies (EBs)-based method. However, currently hESCs are grown mostly using fully defined media in the absence of a feeder layer. Here we describe a EBs-based protocol that allows multilineage differentiation of hESCs and human induced pluripotent stem cells (hiPSCs) grown on feeder-free conditions.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 9%
Student > Doctoral Student 1 9%
Student > Master 1 9%
Unknown 8 73%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 18%
Unknown 9 82%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 November 2021.
All research outputs
#18,809,260
of 23,310,485 outputs
Outputs from Methods in molecular biology
#8,093
of 13,324 outputs
Outputs of similar age
#312,271
of 434,153 outputs
Outputs of similar age from Methods in molecular biology
#146
of 274 outputs
Altmetric has tracked 23,310,485 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,324 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 274 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.