Chapter title |
Mitochondrial DNA Enrichment for Sensitive Next-Generation Sequencing.
|
---|---|
Chapter number | 28 |
Book title |
Mitochondrial DNA
|
Published in |
Methods in molecular biology, January 2023
|
DOI | 10.1007/978-1-0716-2922-2_28 |
Pubmed ID | |
Book ISBNs |
978-1-07-162921-5, 978-1-07-162922-2
|
Authors |
Wu, Shilan, Longley, Matthew J, Lujan, Scott A, Kunkel, Thomas A, Copeland, William C, Longley, Matthew J., Lujan, Scott A., Kunkel, Thomas A., Copeland, William C. |
Abstract |
Mitochondrial DNA (mtDNA) encodes components essential for cellular respiration. Low levels of point mutations and deletions accumulate in mtDNA during normal aging. However, improper maintenance of mtDNA results in mitochondrial diseases, stemming from progressive loss of mitochondrial function through the accelerated formation of deletions and mutations in mtDNA. To better understand the molecular mechanisms underlying the creation and propagation of mtDNA deletions, we developed the LostArc next-generation DNA sequencing pipeline to detect and quantify rare mtDNA species in small tissue samples. LostArc procedures are designed to minimize PCR amplification of mtDNA and instead achieve enrichment of mtDNA by selective destruction of nuclear DNA. This approach leads to cost-effective, high-depth sequencing of mtDNA with a sensitivity sufficient to identify one mtDNA deletion per million mtDNA circles. Here, we describe detailed protocols for isolation of genomic DNA from mouse tissues, enrichment of mtDNA through enzymatic destruction of linear nuclear DNA, and preparation of libraries for unbiased next-generation sequencing of mtDNA. |
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