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Mitochondrial DNA

Overview of attention for book
Cover of 'Mitochondrial DNA'

Table of Contents

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    Book Overview
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    Chapter 1 Isolation of Functional Mitochondria and Pure mtDNA from Murine Tissues
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    Chapter 2 Isolating Mitochondria, Mitoplasts, and mtDNA from Cultured Mammalian Cells.
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    Chapter 3 Coupling Differential Centrifugation with Exonuclease Treatment and Size Exclusion Chromatography (DIFSEC) for Purification of mtDNA from Mammalian Cells
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    Chapter 4 Isolation and Quality Control of Yeast Mitochondria
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    Chapter 5 Mitochondrial DNA Isolation from Plants.
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    Chapter 6 Visualize the Distribution and Dynamics of Mitochondrial DNA (mtDNA) Nucleoids with Multiple Labeling Strategies
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    Chapter 7 Visualization of mtDNA Using FISH
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    Chapter 8 In Situ Analysis of Mitochondrial DNA Synthesis Using Metabolic Labeling Coupled to Fluorescence Microscopy.
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    Chapter 9 Measurement of Nucleoid Size Using STED Microscopy.
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    Chapter 10 How to Quantify DNA Compaction by TFAM with Acoustic Force Spectroscopy and Total Internal Reflection Fluorescence Microscopy
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    Chapter 11 Assessing TFAM Binding to Human Mitochondrial DNA
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    Chapter 12 Identification of Proximity Interactors of Mammalian Nucleoid Proteins by BioID
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    Chapter 13 Localization of Mitochondrial Nucleoids by Transmission Electron Microscopy Using the Transgenic Expression of the Mitochondrial Helicase Twinkle and APEX2
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    Chapter 14 In Vitro Assays of TWINKLE Function
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    Chapter 15 Rolling Circle Replication and Bypass of Damaged Nucleotides
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    Chapter 16 Studying Mitochondrial Nucleic Acid Synthesis Utilizing Intact Isolated Mitochondria
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    Chapter 17 Functional Assessment of Mitochondrial DNA Maintenance by Depletion and Repopulation Using 2’,3’-Dideoxycytidine in Cultured Cells
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    Chapter 18 Analysis of Mitochondrial DNA Replication by Two-Dimensional Agarose Gel Electrophoresis
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    Chapter 19 Quantitative Analysis of Nucleoside Triphosphate Pools in Mouse Muscle Using Hydrophilic Interaction Liquid Chromatography Coupled with Tandem Mass Spectrometry Detection
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    Chapter 20 Detection of UV-Induced Deletions in Mitochondrial DNA
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    Chapter 21 Determination of the Ribonucleotide Content of mtDNA Using Alkaline Gels
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    Chapter 22 5′-End Mapping in Human Mitochondrial DNA
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    Chapter 23 Manipulation of Murine Mitochondrial DNA Heteroplasmy with mtZFNs
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    Chapter 24 Biolistic Transformation of Chlamydomonas reinhardtii and Saccharomyces cerevisiae Mitochondria
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    Chapter 25 A Method for Precisely Identifying Modifications to Plant Mitochondrial Genomes by mitoTALENs.
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    Chapter 26 Mitochondrial DNA Sequencing and Heteroplasmy Quantification by Next Generation Sequencing
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    Chapter 27 Genomic Strategies in Mitochondrial Diagnostics.
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    Chapter 28 Mitochondrial DNA Enrichment for Sensitive Next-Generation Sequencing.
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    Chapter 29 Single Cell Analysis of Mitochondrial DNA Deletions
Attention for Chapter 9: Measurement of Nucleoid Size Using STED Microscopy.
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Chapter title
Measurement of Nucleoid Size Using STED Microscopy.
Chapter number 9
Book title
Mitochondrial DNA
Published in
Methods in molecular biology, January 2023
DOI 10.1007/978-1-0716-2922-2_9
Pubmed ID
Book ISBNs
978-1-07-162921-5, 978-1-07-162922-2
Authors

Motori, Elisa

Abstract

Mitochondria are equipped with their own DNA (mtDNA), which is packed into structures termed nucleoids . While nucleoids can be visualized in situ by fluorescence microscopy , the advent of super-resolution microscopy , and in particular of stimulated emission depletion (STED), has recently enabled the visualization of nucleoids at sub-diffraction resolution. Super-resolution microscopy has proved an invaluable tool for addressing fundamental questions in mitochondrial biology. In this chapter I describe how to achieve efficient labeling of mtDNA and how to quantify nucleoid diameter using an automated approach in fixed cultured cells by STED microscopy .

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The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 100%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 February 2023.
All research outputs
#16,037,422
of 25,378,284 outputs
Outputs from Methods in molecular biology
#4,737
of 14,183 outputs
Outputs of similar age
#231,839
of 479,477 outputs
Outputs of similar age from Methods in molecular biology
#188
of 722 outputs
Altmetric has tracked 25,378,284 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 14,183 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 479,477 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 722 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.