Chapter title |
Reprogramming a GFP Reporter Gene Subjects It to Complex Lentiviral Gene Regulation
|
---|---|
Chapter number | 5 |
Book title |
Synthetic Gene Networks
|
Published in |
Methods in molecular biology, January 2012
|
DOI | 10.1007/978-1-61779-412-4_5 |
Pubmed ID | |
Book ISBNs |
978-1-61779-411-7, 978-1-61779-412-4
|
Authors |
Frank Notka, Ralf Wagner |
Abstract |
Late human immunodeficiency virus (HIV)-derived RNAs encoding relevant therapeutic targets or promising vaccine compounds, such as the HIV-1 group-specific antigen (Gag), are translocated from the nucleus into the cytoplasm via sophisticated export machinery. Relevant steps include the concerted action of several cis-acting RNA elements with the viral Rev-shuttle protein and several cellular components (Ran1/Exportin; Crm1). Based on detailed understanding of the molecular mechanisms guiding this complex process, we used rational codon usage modification to design and reprogram a GFP encoding reporter RNA now exactly mimicking the complex transcriptional and posttranscriptional regulation of late lentiviral mRNAs. |
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