Chapter title |
Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research.
|
---|---|
Chapter number | 6 |
Book title |
Plant Hormones
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6469-7_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6467-3, 978-1-4939-6469-7
|
Authors |
Daniela Ristova, Wolfgang Busch |
Editors |
Jürgen Kleine-Vehn, Michael Sauer |
Abstract |
Genome-wide association (GWA) mapping is a powerful method for the identification of alleles that underlie quantitative traits. It enables one to understand how genetic variation translates into phenotypic variation. In particular, plant hormone signaling pathways play a key role in shaping phenotypes. This chapter presents a protocol for genome-wide association mapping of root traits of Arabidopsis thaliana in the context of hormone research. We describe a specific protocol for acquiring primary and lateral root trait data that is appropriate for GWA studies using FIJI (ImageJ), and subsequent GWA mapping using a user-friendly Internet application. |
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